Effects of granule-associated protein PhaP on glycerol-dependent growth and polymer production in poly(3-hydroxybutyrate)-producing Escherichia coli

 

Autores
<div class="autor_fcen" id="2305">De Almeida, A.</div>; Nikel, P.I.; Giordano, A.M.; <div class="autor_fcen" id="6704">Pettinari, M.J.</div>
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2007
País
Argentina
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
Repositorio
Biblioteca Digital (UBA-FCEN)
Descripción
Polyhydroxyalkanoates (PHAs) are accumulated as intracellular granules by many bacteria under unfavorable conditions, enhancing their fitness and stress resistance. Poly(3-hydroxybutyrate) (PHB) is the most widespread and best-known PHA. Apart from the genes that catalyze polymer biosynthesis, natural PHA producers have several genes for proteins involved in granule formation and/or with regulatory functions, such as phasins, that have been shown to affect polymer synthesis. This study evaluates the effect of PhaP, a phasin, on bacterial growth and PHB accumulation from glycerol in bioreactor cultures of recombinant Escherichia coli carrying phaBAC from Azotobacter sp. strain FA8. Cells expressing phaP grew more, and accumulated more PHB, both using glucose and using glycerol as carbon sources. When cultures were grown in a bioreactor using glycerol, PhaP-bearing cells produced more polymer (2.6 times) and more biomass (1.9 times) than did those without the phasin. The effect of this protein on growth promotion and polymer accumulation is expected to be even greater in high-density cultures, such as those used in the industrial production of the polymer. The recombinant strain presented in this work has been successfully used for the production of PHB from glycerol in bioreactor studies, allowing the production of 7.9 g/liter of the polymer in a semisynthetic medium in 48-h batch cultures. The development of bacterial strains that can efficiently use this substrate can help to make the industrial production of PHAs economically feasible. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Idioma
inglés
OAI Identifier
snrd:HASH04e20f1b7fd4914638db89
Enlace del recurso
http://digital.bl.fcen.uba.ar/Download/paper/paper_00992240_v73_n24_p7912_DeAlmeida.pdf
Nivel de acceso
Acceso abierto
Materia
Bacteria
Biomass
Bioreactors
Biosynthesis
Escherichia coli
Glycerol
Intracellular granules
Polyhydroxyalkanoates (PHAs)
Semisynthetic medium
Stress resistance
Proteins
bacterial protein
carbon
glucose
glycerol
poly(3 hydroxybutyric acid)
polyhydroxyalkanoic acid
polymer
regulator protein
bacterium
fitness
gene
growth
polymer
protein
stress resistance
article
Azotobacter
bacterial growth
bacterial strain
bacterium culture
bioreactor
biosynthesis
catalysis
cell granule
controlled study
Escherichia coli
nonhuman
protein expression
protein function
stress
Azotobacter
Bacterial Proteins
Biomass
Bioreactors
DNA-Binding Proteins
Escherichia coli
Fermentation
Glucose
Glycerol
Hydroxybutyrates
Polyesters
Azotobacter
Bacteria (microorganisms)
Escherichia coli