Pertussis maternal immunization: Narrowing the knowledge gaps on the duration of transferred protective immunity and on vaccination frequency

 

Autores
Gaillard, María Emilia; Bottero, Daniela; Zurita, Maria Eugenia; Carriquiriborde, Francisco Pablo; Martin Aispuro, Pablo; Bartel, Erika Belén; Sabater Martínez, David; Bravo, María Sol; Castuma, Celina; Hozbor, Daniela Flavia
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2017
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Maternal safety through pertussis vaccination and subsequent maternal-fetal-antibody transfer are well documented, but information on infant protection from pertussis by such antibodies and by subsequent vaccinations is scarce. Since mice are used extensively for maternal-vaccination studies, we adopted that model to narrow those gaps in our understanding of maternal pertussis immunization. Accordingly, we vaccinated female mice with commercial acellular pertussis (aP) vaccine and measured offspring protection against Bordetella pertussis challenge and specific-antibody levels with or without revaccination. Maternal immunization protected the offspring against pertussis, with that immune protection transferred to the offspring lasting for several weeks, as evidenced by a reduction (4-5 logs, p < 0.001) in the colony-forming-units recovered from the lungs of 16-week-old offspring. Moreover, maternal-vaccination-acquired immunity from the first pregnancy still conferred protection to offspring up to the fourth pregnancy. Under the conditions of our experimental protocol, protection to offspring from the aP-induced immunity is transferred both transplacentally and through breastfeeding. Adoptive-transfer experiments demonstrated that transferred antibodies were more responsible for the protection detected in offspring than transferred whole spleen cells. In contrast to reported findings, the protection transferred was not lost after the vaccination of infant mice with the same or other vaccine preparations, and conversely, the immunity transferred from mothers did not interfere with the protection conferred by infant vaccination with the same or different vaccines. These results indicated that aP-vaccine immunization of pregnant female mice conferred protective immunity that is transferred both transplacentally and via offspring breastfeeding without compromising the protection boostered by subsequent infant vaccination. These results-though admittedly not necessarily immediately extrapolatable to humans-nevertheless enabled us to test hypotheses under controlled conditions through detailed sampling and data collection. These findings will hopefully refine hypotheses that can then be validated in subsequent human studies.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/53563
Enlace del recurso
http://hdl.handle.net/11336/53563
Nivel de acceso
Acceso abierto
Materia
ACELLULAR VACCINE
BORDETELLA PERTUSSIS
PERTUSSIS
PREGNANCY IMMUNIZATION
PROTECTION
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS