Thermotolerance responses in ripening berries of Vitis vinifera L. cv Muscat Hamburg

 

Autores
Carbonell-Bejerano, Pablo; Santa María, Eva; Torres Perez, Rafael; Royo, Carolina; Lijavetzky, Diego Claudio; Bravo, Gema; Aguirreolea, Jone; Sánchez Diaz, Manuel; Antolin, M.Carmen; Martínez-Zapater, José M.
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2013
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Berry organoleptic properties are highly influenced by ripening environmental conditions. In this study, we used grapevine fruiting cuttings to follow berry ripening under different controlled conditions of temperature and irradiation intensity. Berries ripened at higher temperatures showed reduced anthocyanin accumulation and hastened ripening, leading to a characteristic drop in malic acid and total acidity. The GrapeGen GeneChip combined with a newly developed GrapeGen 12Xv1 MapMan version were utilized for the functional analysis of berry transcriptomic differences after 2 week treatments from veraison onset. These analyses revealed the establishment of a thermotolerance response in berries under high temperatures marked by the induction of heat shock protein (HSP) chaperones and the repression of transmembrane transporter-encoding transcripts. The thermotolerance response was coincident with up-regulation of ERF subfamily transcription factors and increased ABA levels, suggesting their participation in the maintenance of the acclimation response. Lower expression of amino acid transporter-encoding transcripts at high temperature correlated with balanced amino acid content, suggesting a transcriptional compensation of temperature effects on protein and membrane stability to allow for completion of berry ripening. In contrast, the lower accumulation of anthocyanins and higher malate metabolization measured under high temperature might partly result from imbalance in the expression and function of their specific transmembrane transporters and expression changes in genes involved in their metabolic pathways. These results open up new views to improve our understanding of berry ripening under high temperatures.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/2284
Enlace del recurso
http://hdl.handle.net/11336/2284
Nivel de acceso
Acceso abierto
Materia
ANTHOCYANIN
BERRY
MICROARRAY
VITIS VINIFERA
ABA
BERRY RIPENING
LIGHT
TEMPERATURE
TRANSCRIPTOMICS
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS