Modulation of Dendritic Cell Apoptosis and CD8 + Cytotoxicity by Histamine: Role of Protein Kinase C

 

Autores
Alcain, Julieta María; Podaza, Enrique Arturo; Gori, María Soledad; Salamone, Gabriela Veronica; Vermeulen, Elba Monica
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2017
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Dendritic cells (DC) are able to present extracellular antigens associated with the molecules of the major histocompatibility complex class I. In a previous work, we demonstrated that the histamine (HIS), acting through H1/H4 receptors, increases the cross-presentation of soluble ovalbumin by murine DC and can to enhance the recruitment of specific CD8+ T lymphocytes during the development of chronic inflammatory responses. Here, we studied in more depth the mechanisms underlying this enhancement. We showed that the cytotoxicity of specific CD8+ lymphocytes is increased in HIS-treated DC and it is lost by inhibition of vacuolar-ATPase that prevents endosome acidification. It is known that HIS acts through G-protein coupled receptors. The H1/H4 receptors are associated with a Gq subunit, which involves PKC signaling, a pathway related to the apoptotic process. Interestingly, we demonstrated for the first time that HIS prevents DC apoptosis induced by heat-shock through the inhibition of caspase-3, a mechanismdependent on PKC activation, since it is reversed by its inhibition. By contrast, cytolytic activity of T lymphocytes induced by HIS-stimulated DC was independent of PKC pathway.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/40967
Enlace del recurso
http://hdl.handle.net/11336/40967
Nivel de acceso
Acceso abierto
Materia
DENDRITIC CELLS
HISTAMINE
PROTEIN KINASE C
CYTOTOXIC T LYMPHOCYTES
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD