Monofluorophosphate Blocks Internal Polysaccharide Synthesis in Streptococcus mutans

 

Autores
Demonte, Ana María Magdalena; Asención Diez, Matías Damián; Naleway, Conrad; Iglesias, Alberto Alvaro; Ballicora, Miguel A.
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2017
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Streptococcus mutans is the leading cause of dental caries worldwide by accumulating a glycogen-like internal polysaccharide (IPS) that contributes to cariogenicity when sugars are in excess. Sodium monofluorophosphate (MFP) is an active anticariogenic compound in toothpastes. Herein, we show that MFP inhibits (with an I0.5 of 1.5 mM) the S. mutans ADP-glucose pyrophosphorylase (EC 2.7.7.27), which catalyzes the key step in IPS biosynthesis. Enzyme inhibition by MFP is similar to orthophosphate (Pi), except that the effect caused by MFP is not reverted by fructose-1,6-bisP, as occurs with Pi. Inhibition was correlated with a decrease in acidogenesis and IPS accumulation in S. mutans cells cultured with 2 mM sodium MFP. These effects were not mimicked by sodium fluoride. Considering that glycogen synthesis occurs by different pathways in mammals and bacteria, ADP-glucose pyrophosphorylase could be visualized as a molecular target for controlling S. mutans virulence. Our results strongly suggest that MFP is a suitable compound to affect such a target, inducing an anticariogenic effect primarily by inhibiting a key step in IPS synthesis.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/46430
Enlace del recurso
http://hdl.handle.net/11336/46430
Nivel de acceso
Acceso abierto
Materia
ADP-glucose pyrophosphorylase
Allosteric regulation
Internal polysaccharide
Fructose-1,6-bisP
Toothpaste
Inhibition
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS