Antimicrobial, antioxidant, and cytotoxic activities of Bixa orellana Linn

 

Autores
Ul Islam, S.M. Bakhtiar; Hossain, Khalid; Gomes, Isidore; Gomes, Donald J.; Rahman, Sabita Rezwana; Rahman, Mohammad S.; Rashid, Mohammad A.
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2011
País
Argentina
Institución
Universidad Nacional de La Plata
Repositorio
SEDICI (UNLP)
Descripción
Bixa orellana Linn., commonly known as "lipstick plant", is used in folk medicines to treat infections of microbial origin as well as coloring agents in food stuffs in the LDCs like Bangladesh. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the warm water extract of leaves of B. orellana were evaluated against 25 multidrug resistant (MDR) clinical isolates and 6 food-borne pathogens using the micro-dilution broth method modified to comply with the NCCLS standards. The total phenolic content and antioxidant capacity of warm water, ethanol, and methanol extracts of the seeds and leaves of B. orellana were also evaluated. The brine shrimp lethality assay was conducted to assess the toxicity of the extracts. Except Pseudomonas spp., all the MDR isolates and food-borne pathogens tested were susceptible to the warm water extract of the leaves. The MIC and MBC ranged between 8-256 μg/mL and 16 - 256 μg/mL, respectively. Among the test organisms, Streptococcus spp. and Shigella dysenteriae-1 MJ-84 showed highest susceptibility while Escherichia coli exhibited moderate susceptibility to warm water extract of the leaves. The highest total phenolic content (99.99 mg of GAE/g of extractives) and antioxidant capacity (IC50 value 13 μg/mL) were observed in ethanolic extract of seeds of B. orellana, whereas the IC50 of the reference standard BHT (tert-butyl-1-hydroxytoluene) was 59.2 μg/mL. On the other hand, in the brine shrimp lethality bioassay the methanolic extract of the seeds of B. orellana demonstrated strong cytotoxic activity with IC50 value of 19.3 μg/mL. These results suggest that the extracts of B. orellana possess bioactive compounds
Idioma
inglés
OAI Identifier
oai:sedici.unlp.edu.ar:10915/8271
Enlace del recurso
http://sedici.unlp.edu.ar/handle/10915/8271
http://hdl.handle.net/10915/8271
http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_1_13.pdf
Nivel de acceso
Acceso abierto
Materia
Farmacia
Plantas Medicinales
Técnicas de Química Analítica
antioxidant capacity; Bixa orellana Linn; brine shrimp lethality bioassay; multidrug resistant (MDR) bacteria; total phenolic content