Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study

 

Autores
Ma Jianshe; Zhang, Meiling; Shentu, Yangping; Zhang, Yuqing; Fan, Xiaofang; Gong, Yongsheng
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2011
País
Argentina
Institución
Universidad Nacional de La Plata
Repositorio
SEDICI (UNLP)
Descripción
A sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of diphenhydramine hydrochloride in rabbit plasma was developed and validated. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 50 mm, 3.5 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used to quantification using target fragment ions m/z 255.8 → 166.6 for diphenhydramine hydrochloride and m/z 342.9 → 308.0 for the IS. Calibration plots were linear over the range of 5- 200 ng/mL for diphenhydramine hydrochloride in rabbit plasma. Lower limit of quantitation (LLOQ) for diphenhydramine hydrochloride was 5 ng/mL. RSD of intra-day and inter-day precisions were both less than 10 %. This developed method is successfully used in pharmacokinetic study of diphenhydramine hydrochloride in rabbit.
Idioma
inglés
OAI Identifier
oai:sedici.unlp.edu.ar:10915/8308
Enlace del recurso
http://sedici.unlp.edu.ar/handle/10915/8308
http://hdl.handle.net/10915/8308
http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_1_19.pdf
Nivel de acceso
Acceso abierto
Materia
Farmacia
Etilaminas
Técnicas de Química Analítica
Sangre
diphenhydramine hydrochloride; liquid chromatography-tandem mass spectrometry; pharmacokinetics; plasm