In vitro and In vivo toxicology evaluation to determine suitable biomedical Polymers for development of a papain-containing drug delivery system

 

Autores
Alcântara, Mara T. S.; Velasco, María Valéria R.; Zulli, Gislaine; Lopes, Patrícia S.; Miyamaru, Lígia L.; Bárbara, Maria C.S.; Rogero, Suize O.; Lugão, Ademar B.; Mathor, Monica B.
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2011
País
Argentina
Institución
Universidad Nacional de La Plata
Repositorio
SEDICI (UNLP)
Descripción
Papain has been known by many decades for wounded tissues repair. However, papain stability is not high enough to be commercialized in a stable pharmaceutical form; therefore its use is limited. The strategy to entrap papain into a polymeric matrix to provide an adequate drug delivery system consists of an alternative to this problem. The purpose of this study was to assess in vitro and in vivo four polymers cytotoxicity and ability to cause cutaneous irritation to be applied as a suitable papain delivery system. A Monocomponent (MSD) and Bicomponent Silicone Dispersions (BSD) and, Natural Rubber Bicentrifuged Latex (NRBL) and an Acrylic Adhesive (AA) were selected. The cytotoxicity was firstly assessed by the Neutral Red Uptake Method. Non-cytotoxic polymers were then submitted to in vivo Cutaneous Irritation Test. Both silicone dispersions were found non-cytotoxic, and NRBL and AA polymers showed cytotoxicity. MSD and BSD polymers did not cause any cutaneous reactions.
Idioma
inglés
OAI Identifier
oai:sedici.unlp.edu.ar:10915/8268
Enlace del recurso
http://sedici.unlp.edu.ar/handle/10915/8268
http://hdl.handle.net/10915/8268
http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_1_10.pdf
Nivel de acceso
Acceso abierto
Materia
Farmacia
Enzimas
Pruebas de Toxicidad
cutaneous irritation test; cytotoxicity test; drug delivery system; papain