Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens

 

Autores
Valansi, Clari; Moi, David; Leikina, Evgenia; Matveev, Elena; Graña, Martín; Chernomordik, Leonid V.; Romero, Héctor; Aguilar, Pablo Sebastián; Podbilewicz, Benjamin
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2017
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Cell-cell fusion is inherent to sexual reproduction. Loss of HAP LESS 2/GEN ERA TIVE CELL SPE CIF IC 1 (HAP2/GCS1) proteins results in gamete fusion failure in diverse organisms, but their exact role is unclear. In this study, we show that Arabidopsis thaliana HAP2/GCS1 is sufficient to promote mammalian cell-cell fusion. Hemifusion and complete fusion depend on HAP2/GCS1 presence in both fusing cells. Furthermore, expression of HAP2 on the surface of pseudotyped vesicular stomatitis virus results in homotypic virus-cell fusion. We demonstrate that the Caenorhabditis elegans Epithelial Fusion Failure 1 (EFF-1) somatic cell fusogen can replace HAP2/GCS1 in one of the fusing membranes, indicating that HAP2/GCS1 and EFF-1 share a similar fusion mechanism. Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and viral class II fusion proteins (e.g., Zika virus). We name this superfamily Fusexins: fusion proteins essential for sexual reproduction and exoplasmic merger of plasma membranes. We suggest a common origin and evolution of sexual reproduction, enveloped virus entry into cells, and somatic cell fusion.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/53425
Enlace del recurso
http://hdl.handle.net/11336/53425
Nivel de acceso
Acceso abierto
Materia
FUSION
FERTILIZATION
VIRAL
GAMETE
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS