Studies on the participation of epididymal sperm protein DE/CRISP-1 in egg activation

 

Autores
Busso, D.; Cohen, Debora Juana; Da Ros, Vanina Gabriela; Fissore, R.; Cuasnicu, Patricia Sara
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2003
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Protein DE (32 kDa) associates with sperm during epididymal maturation and participates in sperm-egg fusion through its binding to complementary sites on the egg surface. In the present work we investigated the participation of DE in two mechanisms probably involved in egg activation: the ability of DE to trigger activation by its interaction with the binding sites on the egg surface (receptor model) and its ability to regulate intracellular calcium channels (sperm factor model). The incubation of eggs with DE did not promote activation parameters such as calcium oscillations or meiosis resumption. Secondly, microinjection of DE into eggs was ineffective in either eliciting calcium release or modifying oscillations induced by an activating sperm extract. Together, these results argue against the participation of DE in egg activation, restricting the activity of this protein and its egg binding sites to the sperm-egg fusion process.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/36271
Enlace del recurso
http://hdl.handle.net/11336/36271
Nivel de acceso
Acceso abierto
Materia
FERTILIZATION
OOCYTES
EPIDIDYMAL SECRETORY PROTEINS
SPERM CAPACITATION
SPERMATOZOA
Biología Reproductiva
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Bioquímica y Biología Molecular
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD