In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties

 

Autores
Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; Pistone, G.; Volpintesta, R.; Palatnik, S.; Rasia, M.
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2010
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/13757
Enlace del recurso
http://hdl.handle.net/11336/13757
Nivel de acceso
Acceso abierto
Materia
Hyaluronic acid
erythrocyte deformability
erythrocyte aggregation
rheumatoid arthritis
Biofísica
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS