Recombinant protein expression in Escherichia coli: advances and challenges

 

Autores
Rosano, German Leandro; Ceccarelli, Eduardo Augusto
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2014
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/8821
Enlace del recurso
http://hdl.handle.net/11336/8821
Nivel de acceso
Acceso abierto
Materia
Expresión de proteínas
Proteínas heterólogas
Plásmidos de expresión
Escherichia coli
Recombinant protein expression
Expression plasmid
Inclusion bodies
Affinity tags
E. coli expression strains
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS