Non-malignant leukocytes delay spontaneous B-CLL cell apoptosis

 

Autores
Gamberale, Romina; Geffner, Jorge Raúl; Arrosagaray, G.; Scolnik, M.; Salamone, Gabriela Veronica; Trevani, Analía Silvina; Vermeulen, Elba Monica; Giordano, Mirta Nilda
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2001
País
Argentina
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio
CONICET Digital (CONICET)
Descripción
Malignant B cells from chronic lymphocytic leukemia (B-CLL) patients have a long survival in vivo, although, in culture, they spontaneously die by apoptosis. Here, we analyzed the capacity of accessory leukocytes to modulate apoptosis of B-CLL cells in vitro. To this end, we performed long-term cultures using total mononuclear cells (TMC) from B-CLL patients and TMC depleted from monocytes, NK cells and T lymphocytes (B-CLL cells). In all the patients studied (n = 25) the presence of accessory leukocytes markedly prolonged the survival of B-CLL cells. The anti-apoptotic effect was exerted by monocytes and, to a lesser degree, NK cells, partially through the release of soluble factors. Indeed, accessory leukocytes separated from leukemic cells by semipermeable membranes were still able to prolong B-CLL cell survival. By flow cytometric analysis we found that the protective effect of non-malignant cells was associated with delayed down-regulation of Bcl-2 expression on leukemic cells. By contrast, the expression of Fas and Fas ligand proteins was unchanged in most samples. Our findings suggest that monocytes and NK cells, by delaying leukemic cell apoptosis, may play a role in B-CLL cell accumulation in vivo.
Idioma
inglés
OAI Identifier
oai:ri.conicet.gov.ar:11336/55232
Enlace del recurso
http://hdl.handle.net/11336/55232
Nivel de acceso
Acceso abierto
Materia
APOPTOSIS
B-CLL
BCL-2
FAS/FASL
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD