Uterine NK Cells Are Critical in Shaping DC Immunogenic Functions Compatible with Pregnancy Progression

 

Autores
González, I.T.; Barrientos, G.; Freitag, N.; Otto, T.; Thijssen, V.L.J.L.; Moschansky, P.; von Kwiatkowski, P.; Klapp, B.F.; Winterhager, E.; Bauersachs, S.; Blois, S.M.
Tipo de recurso
artículo
Estado
Versión publicada
Año de publicación
2012
País
Argentina
Institución
Repositorio
Descripción
Dendritic cell (DC) and natural killer (NK) cell interactions are important for the regulation of innate and adaptive immunity, but their relevance during early pregnancy remains elusive. Using two different strategies to manipulate the frequency of NK cells and DC during gestation, we investigated their relative impact on the decidualization process and on angiogenic responses that characterize murine implantation. Manipulation of the frequency of NK cells, DC or both lead to a defective decidual response characterized by decreased proliferation and differentiation of stromal cells. Whereas no detrimental effects were evident upon expansion of DC, NK cell ablation in such expanded DC mice severely compromised decidual development and led to early pregnancy loss. Pregnancy failure in these mice was associated with an unbalanced production of anti-angiogenic signals and most notably, with increased expression of genes related to inflammation and immunogenic activation of DC. Thus, NK cells appear to play an important role counteracting potential anomalies raised by DC expansion and overactivity in the decidua, becoming critical for normal pregnancy progression. © 2012 González et al.
Idioma
inglés
OAI Identifier
3573542d-782b-44a2-b2fd-eb61bd043713/snrd:HASH017c8da8fa816d4b4a137469
Enlace del recurso
http://digital.bl.fcen.uba.ar/Download/paper/paper_19326203_v7_n10_p_Gonzalez.pdf
Nivel de acceso
Acceso abierto
Materia
angiopoietin 1
glycoprotein p 15095
animal cell
animal tissue
article
cell differentiation
cell expansion
cell interaction
cell proliferation
cell shape
controlled study
decidualization
dendritic cell
female
first trimester pregnancy
flow cytometry
fluorescence activated cell sorting
gene expression
histopathology
immunogenicity
immunohistochemistry
inflammation
mouse
natural killer cell
nidation
nonhuman
pregnancy
pregnancy disorder
quantitative analysis
reverse transcription polymerase chain reaction
stroma cell
T cell depletion
uterine cervix
Animals
Dendritic Cells
Enzyme-Linked Immunosorbent Assay
Female
Immunohistochemistry
Killer Cells, Natural
Male
Mice
Oligonucleotide Array Sequence Analysis
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
Uterus