In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows

Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO)...

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Autores: Buldain, Daniel Cornelio, Gortari Castillo, Lihuel, Buchamer, Andrea Veronica, Bandoni, Arnaldo Luis, Marchetti, Maria Laura, Mestorino, Nora
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/223246
Acesso em linha:http://hdl.handle.net/11336/223246
Access Level:acceso abierto
Palavra-chave:ERYTHROMYCIN
ESSENTIAL OIL
MASTITIS
MELALEUCA ARMILLARIS
STAPHYLOCOCCUS AUREUS
SYNERGISM
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
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spelling In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows Buldain, Daniel Cornelio Gortari Castillo, Lihuel Buchamer, Andrea Veronica Bandoni, Arnaldo Luis Marchetti, Maria Laura Mestorino, Nora ERYTHROMYCIN ESSENTIAL OIL MASTITIS MELALEUCA ARMILLARIS STAPHYLOCOCCUS AUREUS SYNERGISM https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus. Fil: Buldain, Daniel Cornelio. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Gortari Castillo, Lihuel. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Buchamer, Andrea Veronica. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Fil: Bandoni, Arnaldo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina Fil: Marchetti, Maria Laura. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina Fil: Mestorino, Nora. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Laboratorio de Estudios Farmacologicos y Toxicologicos.; Argentina Frontiers Media http://hdl.handle.net/11336/223246 Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Bandoni, Arnaldo Luis; Marchetti, Maria Laura; et al.; In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows; Frontiers Media; Frontiers in Veterinary Science; 9; 11-2022; 1-11 2297-1769 CONICET Digital CONICET
title In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
spellingShingle In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
Buldain, Daniel Cornelio
ERYTHROMYCIN
ESSENTIAL OIL
MASTITIS
MELALEUCA ARMILLARIS
STAPHYLOCOCCUS AUREUS
SYNERGISM
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
title_short In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_full In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_fullStr In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_full_unstemmed In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
title_sort In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows
author Buldain, Daniel Cornelio
author_facet Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Buchamer, Andrea Veronica
Bandoni, Arnaldo Luis
Marchetti, Maria Laura
Mestorino, Nora
author_role author
author2 Gortari Castillo, Lihuel
Buchamer, Andrea Veronica
Bandoni, Arnaldo Luis
Marchetti, Maria Laura
Mestorino, Nora
author2_role author
author
author
author
author
topic ERYTHROMYCIN
ESSENTIAL OIL
MASTITIS
MELALEUCA ARMILLARIS
STAPHYLOCOCCUS AUREUS
SYNERGISM
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
topic_facet ERYTHROMYCIN
ESSENTIAL OIL
MASTITIS
MELALEUCA ARMILLARIS
STAPHYLOCOCCUS AUREUS
SYNERGISM
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
description Staphylococcus aureus frequently causes subclinical mastitis around the world with a high impact on the milk industry and public health. Essential oils (EO) are recognized antimicrobials that can be synergistic with antibiotics. The main objective of this study was to evaluate the essential oil (EO) of Melaleuca armillaris as an adjuvant of erythromycin (ERY) for the alternative treatment of bovine mastitis caused by S. aureus. The Minimum Inhibitory and Bactericidal Concentrations (MIC and MBC) of EO, ERY, and its combinations were established against S. aureus at different pHs (7.4, 6.5 and 5.0), emulating extra and intracellular conditions. Sensitive (N = 3) and resistant (N = 3) strains to ERY and S. aureus ATCC 29213 as control were used. Math models were applied to describe the antibacterial activity of EO and combinations EO-ERY. The EO was bactericidal against all the strains independently of the pH with a slight improvement in acid conditions. The synergism between EO and ERY was estimated by the Fractional Inhibitory Concentration Index (FIC) and by mathematical modeling of the bacterial killing data. Synergism was observed with ERY, where combinations had bactericidal activity also even with pH modification. M. armillaris EO is an interesting adjuvant for ERY, being a promissory option for further analysis of intracellular efficacy against S. aureus.
publishDate 2022
format article
status_str publishedVersion
url http://hdl.handle.net/11336/223246
identifier_str_mv Buldain, Daniel Cornelio; Gortari Castillo, Lihuel; Buchamer, Andrea Veronica; Bandoni, Arnaldo Luis; Marchetti, Maria Laura; et al.; In vitro synergistic interaction between Melaleuca armillaris essential oil and erythromycin against Staphylococcus aureus isolated from dairy cows; Frontiers Media; Frontiers in Veterinary Science; 9; 11-2022; 1-11
2297-1769
CONICET Digital
CONICET
language eng
eu_rights_str_mv openAccess
publisher Frontiers Media
institution Consejo Nacional de Investigaciones Científicas y Técnicas
collection CONICET Digital (CONICET)
reponame_str CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
_version_ 1878402943674220544
publishDateSort 2022
author_browse Bandoni, Arnaldo Luis
Buchamer, Andrea Veronica
Buldain, Daniel Cornelio
Gortari Castillo, Lihuel
Marchetti, Maria Laura
Mestorino, Nora
publisherStr Frontiers Media
score 6,924472

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