Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β
The expression of arginine-vasopressin (AVP) is regulated by estradiol and testosterone in different neuronal populations by mechanisms that are not yet fully understood. Estrogen receptors (ERs)have been shown to participate in the regulation of AVP neurons by estradiol. In addition, there is evide...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Idioma: | inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/24109 |
| Acceso en línea: | http://hdl.handle.net/11336/24109 |
| Access Level: | acceso abierto |
| Palabra clave: | Argipressin Testosterone Exertion Estrogen Estradiol Aromatase Neurons Oxidoreductase Rna Messenger Phenol Agonists Antagonists Neuroblastoma Cell Metabolites https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
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Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β Grassi, Daniela Bellini, Maria Jose Acaz Fonseca, Estefania Panzica, GianCarlo Garcia Segura, Luis Miguel Argipressin Testosterone Exertion Estrogen Estradiol Aromatase Neurons Oxidoreductase Rna Messenger Phenol Agonists Antagonists Neuroblastoma Cell Metabolites https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 The expression of arginine-vasopressin (AVP) is regulated by estradiol and testosterone in different neuronal populations by mechanisms that are not yet fully understood. Estrogen receptors (ERs)have been shown to participate in the regulation of AVP neurons by estradiol. In addition, there is evidence of the participation of ER in the regulation of AVP expression exerted by testosterone via its metabolite 5- alpha-dihyihydrotestosterone (5-alpha DHT) and its further conversion in the androgen metabolite and ER ligand 3-diol. In this study we have explored the role of ERs in the regulation exerted by estradiol and testosterone on AVP expression, using the human neuroblastoma cell line SH-SY5Y. Estradiol treatment increased AVP mRNA levels in SH-SY5Y cells in comparison to cells treated with vehicle. The stimulatory effect of estradiol on AVP expression was imitated by the ER agonist PPT and blocked by the ER antagonist, ICI 182,780 and the ER antagonist MPP. In contrast, the ER beta agonist DPN reduced AVP expression, while the ER antagonist PHTPP enhanced the action of estradiol on AVP expression. Testosterone increased AVP expression in SH-SY5Y cells by a mechanism that was dependent on aromatase, but not on 5-reducatse activity. testosterone effect was not affected by blocking androgen receptor, was not imitated by the testosterone metabolite 5-DHT, and was blocked by the ER alpha antagonist MPP. In contrast, 5-alpha-DHT had a similar effect than the ER beta agonists DPN and 3-beta-diol, reducing AVP expression. These findings suggest that estradiol and testosterone regulate AVP expression in SH-SY5Y cells through ERs, exerting a stimulatory action via ER alpha and an inhibitory action via ER beta Fil: Grassi, Daniela. Instituto Cajal; España. Università di Torino; Italia Fil: Bellini, Maria Jose. Instituto Cajal; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina Fil: Acaz Fonseca, Estefania. Instituto Cajal; España Fil: Panzica, GianCarlo. Università di Torino; Italia. Neuroscience Institute Cavalieri-Ottolenghi ; Italia Fil: Garcia Segura, Luis Miguel. Instituto Cajal; España Endocrine Society http://hdl.handle.net/11336/24109 Grassi, Daniela; Bellini, Maria Jose; Acaz Fonseca, Estefania; Panzica, GianCarlo; Garcia Segura, Luis Miguel; Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β; Endocrine Society; Endocrinology; 154; 6; 1-6-2013; 2092-2100 0013-7227 CONICET Digital CONICET |
| title |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β |
| spellingShingle |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β Grassi, Daniela Argipressin Testosterone Exertion Estrogen Estradiol Aromatase Neurons Oxidoreductase Rna Messenger Phenol Agonists Antagonists Neuroblastoma Cell Metabolites https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| title_short |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β |
| title_full |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β |
| title_fullStr |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β |
| title_full_unstemmed |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β |
| title_sort |
Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β |
| author |
Grassi, Daniela |
| author_facet |
Grassi, Daniela Bellini, Maria Jose Acaz Fonseca, Estefania Panzica, GianCarlo Garcia Segura, Luis Miguel |
| author_role |
author |
| author2 |
Bellini, Maria Jose Acaz Fonseca, Estefania Panzica, GianCarlo Garcia Segura, Luis Miguel |
| author2_role |
author author author author |
| topic |
Argipressin Testosterone Exertion Estrogen Estradiol Aromatase Neurons Oxidoreductase Rna Messenger Phenol Agonists Antagonists Neuroblastoma Cell Metabolites https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| topic_facet |
Argipressin Testosterone Exertion Estrogen Estradiol Aromatase Neurons Oxidoreductase Rna Messenger Phenol Agonists Antagonists Neuroblastoma Cell Metabolites https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
| description |
The expression of arginine-vasopressin (AVP) is regulated by estradiol and testosterone in different neuronal populations by mechanisms that are not yet fully understood. Estrogen receptors (ERs)have been shown to participate in the regulation of AVP neurons by estradiol. In addition, there is evidence of the participation of ER in the regulation of AVP expression exerted by testosterone via its metabolite 5- alpha-dihyihydrotestosterone (5-alpha DHT) and its further conversion in the androgen metabolite and ER ligand 3-diol. In this study we have explored the role of ERs in the regulation exerted by estradiol and testosterone on AVP expression, using the human neuroblastoma cell line SH-SY5Y. Estradiol treatment increased AVP mRNA levels in SH-SY5Y cells in comparison to cells treated with vehicle. The stimulatory effect of estradiol on AVP expression was imitated by the ER agonist PPT and blocked by the ER antagonist, ICI 182,780 and the ER antagonist MPP. In contrast, the ER beta agonist DPN reduced AVP expression, while the ER antagonist PHTPP enhanced the action of estradiol on AVP expression. Testosterone increased AVP expression in SH-SY5Y cells by a mechanism that was dependent on aromatase, but not on 5-reducatse activity. testosterone effect was not affected by blocking androgen receptor, was not imitated by the testosterone metabolite 5-DHT, and was blocked by the ER alpha antagonist MPP. In contrast, 5-alpha-DHT had a similar effect than the ER beta agonists DPN and 3-beta-diol, reducing AVP expression. These findings suggest that estradiol and testosterone regulate AVP expression in SH-SY5Y cells through ERs, exerting a stimulatory action via ER alpha and an inhibitory action via ER beta |
| publishDate |
2013 |
| format |
article |
| status_str |
publishedVersion |
| url |
http://hdl.handle.net/11336/24109 |
| identifier_str_mv |
Grassi, Daniela; Bellini, Maria Jose; Acaz Fonseca, Estefania; Panzica, GianCarlo; Garcia Segura, Luis Miguel; Estradiol and Testosterone Regulate Arginine-Vasopressin Expression in SH-SY5Y Human Female Neuroblastoma Cells Through Estrogen Receptors-α and -β; Endocrine Society; Endocrinology; 154; 6; 1-6-2013; 2092-2100 0013-7227 CONICET Digital CONICET |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| publisher |
Endocrine Society |
| institution |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| collection |
CONICET Digital (CONICET) |
| reponame_str |
CONICET Digital (CONICET) |
| instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
| _version_ |
1878403173465456640 |
| publishDateSort |
2013 |
| author_browse |
Acaz Fonseca, Estefania Bellini, Maria Jose Garcia Segura, Luis Miguel Grassi, Daniela Panzica, GianCarlo |
| publisherStr |
Endocrine Society |
| score |
6,9008884 |