A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets

A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The met...

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Detalles Bibliográficos
Autores: Gregorini, Anabella, Ruiz, María Esperanza, Volonté, Maria Guillermina
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/4880
Acceso en línea:http://hdl.handle.net/11336/4880
Access Level:acceso abierto
Palabra clave:Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
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spelling A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets Gregorini, Anabella Ruiz, María Esperanza Volonté, Maria Guillermina Levothyroxine Sodium Derivative Uv Spectrophotometry High Performance Liquid Chromatography https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses. Fil: Gregorini, Anabella. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina Fil: Ruiz, María Esperanza. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina Fil: Volonté, Maria Guillermina. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Catedra de Control de Calidad de Medicamentos; Argentina SP MAIK Nauka/Interperiodica http://hdl.handle.net/11336/4880 Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-515 1061-9348
title A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
spellingShingle A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
Gregorini, Anabella
Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
title_short A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_full A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_fullStr A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_full_unstemmed A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
title_sort A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets
author Gregorini, Anabella
author_facet Gregorini, Anabella
Ruiz, María Esperanza
Volonté, Maria Guillermina
author_role author
author2 Ruiz, María Esperanza
Volonté, Maria Guillermina
author2_role author
author
topic Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
topic_facet Levothyroxine Sodium
Derivative Uv Spectrophotometry
High Performance Liquid Chromatography
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
description A derivative UV (DUV) spectrophotometric method was developed for the determination of Levothyroxine Sodium (LT4) in tablets of different doses. Quantification was performed using the second derivative of the absorption spectrum at 253 nm (2D253) in methanol : water (50 : 50; v/v) (pH 11.2). The method was validated and compared with an HPLC procedure carried out using a RP18 column (125 x~ 4 mm, 5 microm) and methanol: phosphoric acid (0.1%) (70 : 30, v/v) (pH 3) as mobile phase. Flow rate was set at 1.5 mL/min, and detection was performed at 225 nm. The proposed DUV method was linear in the range 3.0 - 40.0 microg/mL with an appropriate precision and accuracy, and it was selective for the drug under study. On the other hand, results obtained by 2D253 analysis were similar to those obtained by HPLC, with no statistically significant differences between them. Therefore, it was concluded that the developed method is suitable for the determination of LT4 in tablets at the tested doses.
publishDate 2013
format article
status_str publishedVersion
url http://hdl.handle.net/11336/4880
identifier_str_mv Gregorini, Anabella; Ruiz, María Esperanza; Volonté, Maria Guillermina; A derivative UV spectrophotometry method for the determination of levothyroxine sodium in tablets; SP MAIK Nauka/Interperiodica; Journal Of Analytical Chemistry; 68; 6; 6-2013; 510-515
1061-9348
language eng
eu_rights_str_mv openAccess
publisher SP MAIK Nauka/Interperiodica
institution Consejo Nacional de Investigaciones Científicas y Técnicas
collection CONICET Digital (CONICET)
reponame_str CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
_version_ 1878403280056352768
publishDateSort 2013
author_browse Gregorini, Anabella
Ruiz, María Esperanza
Volonté, Maria Guillermina
publisherStr SP MAIK Nauka/Interperiodica
score 6,9008884

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