Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data

Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In t...

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Detalles Bibliográficos
Autores: Debat, Humberto Julio, Garcia, Maria Laura, Bejerman, Nicolas
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Argentina
Institución:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositorio:CONICET Digital (CONICET)
Idioma:inglés
OAI Identifier:oai:ri.conicet.gov.ar:11336/226369
Acceso en línea:http://hdl.handle.net/11336/226369
Access Level:acceso abierto
Palabra clave:METATRANSCRIPTOMICS
OPHIOVIRUS
PLANT VIRUSES
VIRUS DISCOVERY
VIRUS TAXONOMY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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spelling Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data Debat, Humberto Julio Garcia, Maria Laura Bejerman, Nicolas METATRANSCRIPTOMICS OPHIOVIRUS PLANT VIRUSES VIRUS DISCOVERY VIRUS TAXONOMY https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus. Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Bejerman, Nicolas. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina MDPI http://hdl.handle.net/11336/226369 Debat, Humberto Julio; Garcia, Maria Laura; Bejerman, Nicolas; Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data; MDPI; Viruses; 15; 4; 3-2023; 1-21 1999-4915 CONICET Digital CONICET
title Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
spellingShingle Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
Debat, Humberto Julio
METATRANSCRIPTOMICS
OPHIOVIRUS
PLANT VIRUSES
VIRUS DISCOVERY
VIRUS TAXONOMY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
title_full Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
title_fullStr Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
title_full_unstemmed Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
title_sort Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Garcia, Maria Laura
Bejerman, Nicolas
author_role author
author2 Garcia, Maria Laura
Bejerman, Nicolas
author2_role author
author
topic METATRANSCRIPTOMICS
OPHIOVIRUS
PLANT VIRUSES
VIRUS DISCOVERY
VIRUS TAXONOMY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic_facet METATRANSCRIPTOMICS
OPHIOVIRUS
PLANT VIRUSES
VIRUS DISCOVERY
VIRUS TAXONOMY
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description Ophioviruses (genus Ophiovirus, family Aspiviridae) are plant-infecting viruses with non-enveloped, filamentous, naked nucleocapsid virions. Members of the genus Ophiovirus have a segmented single-stranded negative-sense RNA genome (ca. 11.3–12.5 kb), encompassing three or four linear segments. In total, these segments encode four to seven proteins in the sense and antisense orientation, both in the viral and complementary strands. The genus Ophiovirus includes seven species with viruses infecting both monocots and dicots, mostly trees, shrubs and some ornamentals. From a genomic perspective, as of today, there are complete genomes available for only four species. Here, by exploring large publicly available metatranscriptomics datasets, we report the identification and molecular characterization of 33 novel viruses with genetic and evolutionary cues of ophioviruses. Genetic distance and evolutionary insights suggest that all the detected viruses could correspond to members of novel species, which expand the current diversity of ophioviruses ca. 4.5-fold. The detected viruses increase the tentative host range of ophioviruses for the first time to mosses, liverwort and ferns. In addition, the viruses were linked to several Asteraceae, Orchidaceae and Poaceae crops/ornamental plants. Phylogenetic analyses showed a novel clade of mosses, liverworts and fern ophioviruses, characterized by long branches, suggesting that there is still plenty of unsampled hidden diversity within the genus. This study represents a significant expansion of the genomics of ophioviruses, opening the door to future works on the molecular and evolutionary peculiarity of this virus genus.
publishDate 2023
format article
status_str publishedVersion
url http://hdl.handle.net/11336/226369
identifier_str_mv Debat, Humberto Julio; Garcia, Maria Laura; Bejerman, Nicolas; Expanding the Repertoire of the Plant-Infecting Ophioviruses through Metatranscriptomics Data; MDPI; Viruses; 15; 4; 3-2023; 1-21
1999-4915
CONICET Digital
CONICET
language eng
eu_rights_str_mv openAccess
publisher MDPI
institution Consejo Nacional de Investigaciones Científicas y Técnicas
collection CONICET Digital (CONICET)
reponame_str CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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publishDateSort 2023
author_browse Bejerman, Nicolas
Debat, Humberto Julio
Garcia, Maria Laura
publisherStr MDPI
score 6,9460754

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