Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism

The circadian clock is an endogenous system that allows organisms to daily adapt and optimize their physiology and metabolism. We studied the key circadian clock gene (CCG) orthologs in Nicotiana tabacum seedlings and in hairy root cultures (HRC). Putative genes involved in the metabolism of xenobio...

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Autores: Sosa Alderete, Lucas Gastón, Guido, Mario Eduardo, Agostini, Elizabeth, Mas, Paloma
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2018
País:Argentina
Recursos:Consejo Nacional de Investigaciones Científicas y Técnicas
Repositório:CONICET Digital (CONICET)
Idioma:inglês
OAI Identifier:oai:ri.conicet.gov.ar:11336/72816
Acesso em linha:http://hdl.handle.net/11336/72816
Access Level:Acceso aberto
Palavra-chave:Circadian Clock
Phenol Treatment
Tobacco Hairy Roots
Xenobiotic Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72816
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spelling Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism Sosa Alderete, Lucas Gastón Guido, Mario Eduardo Agostini, Elizabeth Mas, Paloma Circadian Clock Phenol Treatment Tobacco Hairy Roots Xenobiotic Metabolism https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 The circadian clock is an endogenous system that allows organisms to daily adapt and optimize their physiology and metabolism. We studied the key circadian clock gene (CCG) orthologs in Nicotiana tabacum seedlings and in hairy root cultures (HRC). Putative genes involved in the metabolism of xenobiotic compounds (MXC) were selected and their expression profiles were also analyzed. Seedlings and HRC displayed similar diurnal variations in the expression profiles for the CCG examined under control conditions (CC). MXC-related genes also showed daily fluctuations with specific peaks of expression. However, when HRC were under phenol treatment (PT), the expression patterns of the clock and MXC-related genes were significantly affected. In 2-week-old HRC, PT downregulated the expression of NtLHY, NtTOC1, and NtPRR9 while NtFKF1 and NtGI genes were upregulated by phenol. In 3-week-old HRC, PT also downregulated the expression of all CCG analyzed and NtTOC1 was the most affected. Following PT, the expression of the MXC-related genes was upregulated or displayed an anti-phasic expression profile compared to the expression under CC. Our studies thus provide a glimpse of the circadian expression of clock genes in tobacco and the use of HRC as a convenient system to study plant responses to xenobiotic stresses. Fil: Sosa Alderete, Lucas Gastón. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Guido, Mario Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Agostini, Elizabeth. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mas, Paloma. Consejo Superior de Investigaciones Científicas; España. Center for Research in Agricultural Genomics; España Springer Heidelberg http://hdl.handle.net/11336/72816 Sosa Alderete, Lucas Gastón; Guido, Mario Eduardo; Agostini, Elizabeth; Mas, Paloma; Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism; Springer Heidelberg; Environmental Science and Pollution Research; 25; 2; 1-2018; 1597-1608 0944-1344 1614-7499 CONICET Digital CONICET
title Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
spellingShingle Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
Sosa Alderete, Lucas Gastón
Circadian Clock
Phenol Treatment
Tobacco Hairy Roots
Xenobiotic Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
title_short Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
title_full Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
title_fullStr Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
title_full_unstemmed Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
title_sort Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism
author Sosa Alderete, Lucas Gastón
author_facet Sosa Alderete, Lucas Gastón
Guido, Mario Eduardo
Agostini, Elizabeth
Mas, Paloma
author_role author
author2 Guido, Mario Eduardo
Agostini, Elizabeth
Mas, Paloma
author2_role author
author
author
topic Circadian Clock
Phenol Treatment
Tobacco Hairy Roots
Xenobiotic Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
topic_facet Circadian Clock
Phenol Treatment
Tobacco Hairy Roots
Xenobiotic Metabolism
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
description The circadian clock is an endogenous system that allows organisms to daily adapt and optimize their physiology and metabolism. We studied the key circadian clock gene (CCG) orthologs in Nicotiana tabacum seedlings and in hairy root cultures (HRC). Putative genes involved in the metabolism of xenobiotic compounds (MXC) were selected and their expression profiles were also analyzed. Seedlings and HRC displayed similar diurnal variations in the expression profiles for the CCG examined under control conditions (CC). MXC-related genes also showed daily fluctuations with specific peaks of expression. However, when HRC were under phenol treatment (PT), the expression patterns of the clock and MXC-related genes were significantly affected. In 2-week-old HRC, PT downregulated the expression of NtLHY, NtTOC1, and NtPRR9 while NtFKF1 and NtGI genes were upregulated by phenol. In 3-week-old HRC, PT also downregulated the expression of all CCG analyzed and NtTOC1 was the most affected. Following PT, the expression of the MXC-related genes was upregulated or displayed an anti-phasic expression profile compared to the expression under CC. Our studies thus provide a glimpse of the circadian expression of clock genes in tobacco and the use of HRC as a convenient system to study plant responses to xenobiotic stresses.
publishDate 2018
format article
status_str publishedVersion
url http://hdl.handle.net/11336/72816
identifier_str_mv Sosa Alderete, Lucas Gastón; Guido, Mario Eduardo; Agostini, Elizabeth; Mas, Paloma; Identification and characterization of key circadian clock genes of tobacco hairy roots: putative regulatory role in xenobiotic metabolism; Springer Heidelberg; Environmental Science and Pollution Research; 25; 2; 1-2018; 1597-1608
0944-1344
1614-7499
CONICET Digital
CONICET
language eng
eu_rights_str_mv openAccess
publisher Springer Heidelberg
institution Consejo Nacional de Investigaciones Científicas y Técnicas
collection CONICET Digital (CONICET)
reponame_str CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
_version_ 1878403907257892864
publishDateSort 2018
author_browse Agostini, Elizabeth
Guido, Mario Eduardo
Mas, Paloma
Sosa Alderete, Lucas Gastón
publisherStr Springer Heidelberg
score 6,924472

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