STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro
Background: LePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2010 |
| Country: | Argentina |
| Institution: | Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales |
| Repository: | Biblioteca Digital (UBA-FCEN) |
| Language: | English |
| OAI Identifier: | paperaa:paper_14712229_v10_n_p_Wengier |
| Online Access: | http://hdl.handle.net/20.500.12110/paper_14712229_v10_n_p_Wengier |
| Access Level: | Open access |
| Keyword: | Lycopersicon esculentum Nicotiana tabacum protein kinase C protein kinase N vegetable protein article genetics growth, development and aging isolation and purification metabolism phosphorylation pollen tube tomato Phosphorylation Plant Proteins Pollen Tube Protein Kinase C |
| Summary: | Background: LePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato or tobacco style extracts.Results: Here we show that LePRK2 dephosphorylation is mediated by a heat-, acid-, base-, DTT- and protease-resistant component from tobacco styles. Using LePRK2 phosphorylation as a tracking assay for purification, style exudates were subjected to chloroform extraction, anionic exchange, and C18 reverse-phase chromatography columns. We finally obtained a single ~3,550 Da compound (as determined by UV-MALDI-TOF MS) that we named STIL (for Style Interactor for LePRKs). STIL increased pollen tube lengths of in vitro germinated pollen in a dose-dependent manner.Conclusion: We propose that the LePRK complex perceives STIL, resulting in LePRK2 dephosphorylation and an increase in pollen tube growth. © 2010 Wengier et al; licensee BioMed Central Ltd. |
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