Papel e regulação da via de processamento de pequenos RNAs em células somáticas do nematoide Caenorhabditis elegans
Aging increases the risk of chronic diseases, and may be delayed by restrictions Caloric. Previously, our group and collaborators have identified that conserved form, the microRNAs synthesis decreases with age - being reversed by the restriction Caloric. This phenomenon is due to the regulation of t...
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| Tipo de documento: | dissertação |
| Estado: | Versão publicada |
| Data de publicação: | 2016 |
| País: | Brasil |
| Recursos: | Universidade Federal de São Paulo (UNIFESP) |
| Repositório: | Repositório Institucional da UNIFESP |
| Idioma: | português |
| OAI Identifier: | oai:repositorio.unifesp.br:11600/46185 |
| Acesso em linha: | https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3686551 http://repositorio.unifesp.br/handle/11600/46185 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Aging Caenorhabdifis elegans Line Germ dicer Rnai Envelhecimento Linhagem germinativa dicer |
| Resumo: | Aging increases the risk of chronic diseases, and may be delayed by restrictions Caloric. Previously, our group and collaborators have identified that conserved form, the microRNAs synthesis decreases with age - being reversed by the restriction Caloric. This phenomenon is due to the regulation of the expression of key enzymes in processing microRNAs, especially Dicer, which cleaves the pre-miRNA in mature microRNA. We seek this work to identify how this pathway components are in C. elegans regulated in situations that affect longevity. Therefore, we use strains integrated into the genome of C. elegans in which Dicer or Argonaut gene-I under control of their respective promoters encode a protein fused to GFP. With the microscopic analysis, we monitored the expression of these proteins in different situations, as caloric restriction, aging and long-lived worms without germline. We found that in the absence of gerrninati va line's activation 'of the route of synthesis microRNAs cm somatic cells. Furthermore, the increase in longevity of these worms dependent on Dicer. To identify regulators of expression of Dicer and Argonaut-l We conducted a genetic scan from analysis of modENCODE (database ChIP-Seq experiments). For Argonauta-L, we can identify that there is regulation by a transcription factor conserved, PE: li \ -4 / FOXA, which operates in calorie restriction. Lastly, we obtained a set of results that are a note of caution vector unrestricted use L4440 control for RNAi transgene study worms where it produces an RNA double-stranded silencing in vivo transgene GFP |
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