Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design
Botryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its c...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2011 |
| País: | Brasil |
| Institución: | Universidade Estadual Paulista (UNESP) |
| Repositorio: | Repositório Institucional da UNESP |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unesp.br:11449/72299 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.bej.2010.10.013 http://hdl.handle.net/11449/72299 |
| Access Level: | acceso abierto |
| Palabra clave: | β-1,3-Glucanases Botryosphaeran Statistical mixture-design optimization Botryosphaeria rhodina MAMB-05 Carbon source Curdlan De-repression Enzyme production Exponential growth Glucanase Laminarins Nutrient medium Synergic effects Trichoderma harzianum Rifai Design Enzymes Fungi Glucose Mixtures Optimization 1,3 beta glucanase curdlan glucose lactose laminaran botryosphaeria rhodina comparative study correlation analysis enzyme substrate enzyme synthesis fungus Hypocrea lixii mycelium nonhuman priority journal statistical analysis Botryosphaeria rhodina |
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Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design Giese, Ellen C. [UNESP] Dekker, Robert F.H. Scarminio, Ieda S. Barbosa, Aneli M. da Silva, Roberto [UNESP] β-1,3-Glucanases Botryosphaeran Statistical mixture-design optimization Botryosphaeria rhodina MAMB-05 Carbon source Curdlan De-repression Enzyme production Exponential growth Glucanase Laminarins Nutrient medium Synergic effects Trichoderma harzianum Rifai Design Enzymes Fungi Glucose Mixtures Optimization 1,3 beta glucanase curdlan glucose lactose laminaran botryosphaeria rhodina comparative study correlation analysis enzyme substrate enzyme synthesis fungus Hypocrea lixii mycelium nonhuman priority journal statistical analysis Botryosphaeria rhodina Botryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its consumption (after 108h), and de-repression of β-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of β-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of β-1,3-glucanases and β-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used to optimize β-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30UmL -1), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for β-glucanase production by T. harzianum (4.25UmL -1). © 2010 Elsevier B.V. Departamento de Química e Ciências Ambientais Universidade Estadual Paulista São José do Rio Preto-SP Biorefining Research Initiative Lakehead University, Thunder Bay-ON Departamento de Química Universidade Estadual de Londrina, Londrina-PR Departamento de Bioquímica e Biotecnologia Universidade Estadual de Londrina, Londrina-PR http://dx.doi.org/10.1016/j.bej.2010.10.013 Biochemical Engineering Journal, v. 53, n. 2, p. 239-243, 2011. 1369-703X http://hdl.handle.net/11449/72299 10.1016/j.bej.2010.10.013 2-s2.0-78649960162 2-s2.0-78649960162.pdf 9424175688206545 |
| title |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design |
| spellingShingle |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design Giese, Ellen C. [UNESP] β-1,3-Glucanases Botryosphaeran Statistical mixture-design optimization Botryosphaeria rhodina MAMB-05 Carbon source Curdlan De-repression Enzyme production Exponential growth Glucanase Laminarins Nutrient medium Synergic effects Trichoderma harzianum Rifai Design Enzymes Fungi Glucose Mixtures Optimization 1,3 beta glucanase curdlan glucose lactose laminaran botryosphaeria rhodina comparative study correlation analysis enzyme substrate enzyme synthesis fungus Hypocrea lixii mycelium nonhuman priority journal statistical analysis Botryosphaeria rhodina |
| title_short |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design |
| title_full |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design |
| title_fullStr |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design |
| title_full_unstemmed |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design |
| title_sort |
Comparison of β-1,3-glucanase production by Botryosphaeria rhodina MAMB-05 and Trichoderma harzianum Rifai and its optimization using a statistical mixture-design |
| author |
Giese, Ellen C. [UNESP] |
| author_facet |
Giese, Ellen C. [UNESP] Dekker, Robert F.H. Scarminio, Ieda S. Barbosa, Aneli M. da Silva, Roberto [UNESP] |
| author_role |
author |
| author2 |
Dekker, Robert F.H. Scarminio, Ieda S. Barbosa, Aneli M. da Silva, Roberto [UNESP] |
| author2_role |
author author author author |
| topic |
β-1,3-Glucanases Botryosphaeran Statistical mixture-design optimization Botryosphaeria rhodina MAMB-05 Carbon source Curdlan De-repression Enzyme production Exponential growth Glucanase Laminarins Nutrient medium Synergic effects Trichoderma harzianum Rifai Design Enzymes Fungi Glucose Mixtures Optimization 1,3 beta glucanase curdlan glucose lactose laminaran botryosphaeria rhodina comparative study correlation analysis enzyme substrate enzyme synthesis fungus Hypocrea lixii mycelium nonhuman priority journal statistical analysis Botryosphaeria rhodina |
| topic_facet |
β-1,3-Glucanases Botryosphaeran Statistical mixture-design optimization Botryosphaeria rhodina MAMB-05 Carbon source Curdlan De-repression Enzyme production Exponential growth Glucanase Laminarins Nutrient medium Synergic effects Trichoderma harzianum Rifai Design Enzymes Fungi Glucose Mixtures Optimization 1,3 beta glucanase curdlan glucose lactose laminaran botryosphaeria rhodina comparative study correlation analysis enzyme substrate enzyme synthesis fungus Hypocrea lixii mycelium nonhuman priority journal statistical analysis Botryosphaeria rhodina |
| description |
Botryosphaeria rhodina MAMB-05 produced β-1,3-glucanases and botryosphaeran when grown on glucose, while Trichoderma harzianum Rifai only produced the enzyme. A comparison of long-term cultivation (300h) by B. rhodina demonstrated a correlation between the formation of botryosphaeran (48h) and its consumption (after 108h), and de-repression of β-1,3-glucanase synthesis when glucose was depleted from the nutrient medium, whereas for T. harzianum enzyme production commenced during exponential growth. Growth profiles and levels of β-1,3-glucanases produced by both fungi on botryosphaeran also differed, as well as the production of β-1,3-glucanases and β-1,6-glucanases on glucose, lactose, laminarin, botryosphaeran, lasiodiplodan, curdlan, Brewer's yeast powder and lyophilized fungal mycelium, which were dependent upon the carbon source used. A statistical mixture-design used to optimize β-1,3-glucanase production by both fungi evaluated botryosphaeran, glucose and lactose concentrations as variables. For B. rhodina, glucose and lactose promoted enzyme production at the same levels (2.30UmL -1), whereas botryosphaeran added to these substrates exerted a synergic effect favorable for β-glucanase production by T. harzianum (4.25UmL -1). © 2010 Elsevier B.V. |
| publishDate |
2011 |
| format |
article |
| status_str |
publishedVersion |
| url |
http://dx.doi.org/10.1016/j.bej.2010.10.013 http://hdl.handle.net/11449/72299 |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| institution |
UNESP |
| collection |
Repositório Institucional da UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| _version_ |
1878763407813902336 |
| publishDateSort |
2011 |
| author_browse |
Barbosa, Aneli M. Dekker, Robert F.H. Giese, Ellen C. [UNESP] Scarminio, Ieda S. da Silva, Roberto [UNESP] |
| score |
6,8972664 |