Mutación del gen KRAS en el cáncer de colon y recto
Background: KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. Aim: To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and Methods: KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal canc...
| Authors: | , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2013 |
| Country: | Chile |
| Institution: | CONICYT Chile |
| Repository: | SciELO Chile |
| OAI Identifier: | oai:scielo:S0034-98872013000900009 |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0034-98872013000900009 |
| Access Level: | Open access |
| Keyword: | Colonic neoplasms Rectal neoplasms Sarcospan (Kras oncogene-associated gene) protein, human |
| Summary: | Background: KRAS oncogene is involved in colorectal carcinogenesis in 22 to 45% of cases. Aim: To determine the frequency, types and distribution of KRAS mutations in colorectal cancer. Material and Methods: KRAS mutations studies were carried out in primary tumors and metastases of colo-rectal cancer from 56 women aged 60 ± 14 years and 53 men aged 61 ± 11 years. Formalin fixed and paraffin embedded tissue samples were evaluated using RFLP (Restriction Fragment Length Polymorphism) and direct sequencing. Results: Primary tumors were located in the colon and rectum in 82 (75.2%) and 24 cases (20%), respectively. In three cases the extraction site of the tumor sample was unknown. In 46 cases (42.2%) KRAS mutations were demonstrated. The main point mutations were located in codon 12 (80.4%), G12D (39.1%), G12V (24.2%), G12S (6.5%), G12A (4.3%); G12C (4.3%), G12R (2.1%) and 19.6% at codon 13 (G13D). No differences were demonstrated in the frequency and distribution of mutations by gender, age, primary versus metastatic tumors or tumor location. Conclusions: In this series, 42% of colorectal cancer tissue samples had KRAS mutations. Their frequency and distribution are similar to those reported in the literature, except for G12C mutation. |
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