In vitro: Effectiveness of Laurus nobilis extract against Hymenolepis nana Eggs
Abstract Parasites are among the most widespread health threats affecting a wide range of organisms. Hymenolepis nana is a common intestinal tapeworm that infects rodents such as Mus musculus and Rattus norvegicus, posing potential complications in laboratory research where animal health is crucial...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | Chile |
| Institución: | Agencia Nacional de Investigación y Desarrollo |
| Repositorio: | SciELO Chile |
| OAI Identifier: | oai:scielo:S2452-57312025000300138 |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S2452-57312025000300138 |
| Access Level: | acceso abierto |
| Palabra clave: | In vitro Hymenolepis nana Laurus nobilis eggs Albendazole |
| Sumario: | Abstract Parasites are among the most widespread health threats affecting a wide range of organisms. Hymenolepis nana is a common intestinal tapeworm that infects rodents such as Mus musculus and Rattus norvegicus, posing potential complications in laboratory research where animal health is crucial for obtaining reliable results. This study aimed to investigate the ovicidal effect of Laurus nobilis leaf extract on H. nana eggs. Eggs were divided into six groups, each containing 500 eggs. Groups 2 to 5 were treated with L. nobilis extract at concentrations of 25, 50, 100, and 200 µg ml-1, respectively, while group 6 received Albendazole at 100 mg ml-1 as a reference drug. A control group was maintained without treatment. All groups were incubated at 28 °C and examined after 12, 24, 36, and 48 hours. Following incubation, a mixture of the egg suspension and 0.1% eosin dye was prepared to assess egg viability under a light microscope. Results showed that the highest concentration of L. nobilis (200 µg ml-1) killed 89% of the eggs, while Albendazole (100 mg ml-1) achieved a 93% mortality rate. Non-viable (dead) eggs absorbed the red dye, while viable eggs remained unstained. Statistically significant differences (P≤0.01) were observed between treated groups and the control, as well as among the different extract concentrations. These findings demonstrate the potent ovicidal activity of L. nobilis, highlighting its potential as a natural antiparasitic agent and a promising candidate for the development of plant-based anthelmintic drugs. |
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