Membrane damage of bacteria by silanols treatment
Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were trea...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | Chile |
| Institución: | Agencia Nacional de Investigación y Desarrollo |
| Repositorio: | SciELO Chile |
| OAI Identifier: | oai:scielo:S0717-34582007000200009 |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009 |
| Access Level: | acceso abierto |
| Palabra clave: | antimicrobials hydrophobicity organosilicon compounds |
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Membrane damage of bacteria by silanols treatmentKim,Yun-miFarrah,SamuelBaney,Ronald Hantimicrobialshydrophobicityorganosilicon compoundsAntimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were treated by silanols at concentration of less than 0.2 wt% for one hour. Membrane damage of the bacteria by the silanol treatment was clearly observed by transmission electron microscopy. Separation of the cytoplasmic membrane from the outer membrane for E. coli and disorganized cytoplasmic membrane of the Gram-positive bacteria were observed when compared to the control. Fluorescent dyes, green-fluorescent nucleic acid stain (Syto 9) and the red-fluorescent nucleic acid stain (Propidium iodide), were used to monitor membrane damage of the bacteria by Confocal microscopy and Spectrophotometer. A reduction of the green fluorescent emission was detected for silanol treated bacteria indicating membrane damage of the bacteria and supporting the hypothesis that their viability loss may be due to their membrane damage analogus to alcoholsPontificia Universidad Católica de Valparaíso2007-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009Electronic Journal of Biotechnology v.10 n.2 2007reponame:SciELO Chileinstname:Agencia Nacional de Investigación y Desarrolloinstacron:ANID-SciELO Chileen10.4067/S0717-34582007000200009info:eu-repo/semantics/openAccessinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2007-10-29T00:00:00Zoai:scielo:S0717-34582007000200009opendoar:2007-10-29T00:00falseopendoar:2007-10-29T00:00SciELO Chile - Agencia Nacional de Investigación y Desarrollofalse |
| dc.title.none.fl_str_mv |
Membrane damage of bacteria by silanols treatment |
| title |
Membrane damage of bacteria by silanols treatment |
| spellingShingle |
Membrane damage of bacteria by silanols treatment Kim,Yun-mi antimicrobials hydrophobicity organosilicon compounds |
| title_short |
Membrane damage of bacteria by silanols treatment |
| title_full |
Membrane damage of bacteria by silanols treatment |
| title_fullStr |
Membrane damage of bacteria by silanols treatment |
| title_full_unstemmed |
Membrane damage of bacteria by silanols treatment |
| title_sort |
Membrane damage of bacteria by silanols treatment |
| dc.creator.none.fl_str_mv |
Kim,Yun-mi Farrah,Samuel Baney,Ronald H |
| author |
Kim,Yun-mi |
| author_facet |
Kim,Yun-mi Farrah,Samuel Baney,Ronald H |
| author_role |
author |
| author2 |
Farrah,Samuel Baney,Ronald H |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
antimicrobials hydrophobicity organosilicon compounds |
| topic |
antimicrobials hydrophobicity organosilicon compounds |
| description |
Antimicrobial action of silanols, a new class of antimicrobials, was investigated by transmission electron microscopy and fluorescent dye studies. Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were treated by silanols at concentration of less than 0.2 wt% for one hour. Membrane damage of the bacteria by the silanol treatment was clearly observed by transmission electron microscopy. Separation of the cytoplasmic membrane from the outer membrane for E. coli and disorganized cytoplasmic membrane of the Gram-positive bacteria were observed when compared to the control. Fluorescent dyes, green-fluorescent nucleic acid stain (Syto 9) and the red-fluorescent nucleic acid stain (Propidium iodide), were used to monitor membrane damage of the bacteria by Confocal microscopy and Spectrophotometer. A reduction of the green fluorescent emission was detected for silanol treated bacteria indicating membrane damage of the bacteria and supporting the hypothesis that their viability loss may be due to their membrane damage analogus to alcohols |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-04-01 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009 |
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200009 |
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en |
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en |
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10.4067/S0717-34582007000200009 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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text/html |
| dc.publisher.none.fl_str_mv |
Pontificia Universidad Católica de Valparaíso |
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Pontificia Universidad Católica de Valparaíso |
| dc.source.none.fl_str_mv |
Electronic Journal of Biotechnology v.10 n.2 2007 reponame:SciELO Chile instname:Agencia Nacional de Investigación y Desarrollo instacron:ANID-SciELO Chile |
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Agencia Nacional de Investigación y Desarrollo |
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ANID-SciELO Chile |
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ANID-SciELO Chile |
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SciELO Chile |
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SciELO Chile |
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SciELO Chile - Agencia Nacional de Investigación y Desarrollo |
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