Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis
11 páginas, 5 figuras.
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/286986 |
| Acceso en línea: | http://hdl.handle.net/10261/286986 |
| Access Level: | acceso abierto |
| Palabra clave: | Paratuberculosis Vaccination Ruminants Mycobacterium avium subspecies paratuberculosis Interferon-gamma Immune response Tuberculosis Antigens |
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oai:digital.csic.es:10261/286986 |
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| network_name_str |
España |
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Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis Fernández Fernández, Miguel Royo González, Marcos Arteche-Villasol, Noive Ferreras, Mª del Carmen Pérez Pérez, Valentín Paratuberculosis Vaccination Ruminants Mycobacterium avium subspecies paratuberculosis Interferon-gamma Immune response Tuberculosis Antigens 11 páginas, 5 figuras. Vaccination can be an efficient method for the control of paratuberculosis in ruminants. However, the official tuberculosis control tests cross-interfere with the animals vaccinated against paratuberculosis. In order to test and compare new antigens that could solve this problem, the production of interferon-gamma (IFN-γ) in peripheral blood at different post-vaccination days in experimental kids and adult goats, in field conditions, using the avian and bovine purified protein derivative (PPD), the johnin, two peptide cocktails of Mycobacterium bovis (PC-EC and PC-HP) and the antigens VK 055 and VK 067 of Mycobacterium avium subspecies paratuberculosis (Map) has been analyzed in vitro. The non-specific production of IFN-γ was observed after blood stimulation with the PC-EC and PC-HP cocktail in any sample from vaccinated animals, whereas it was detected when bovine PPD was used. These results support the possible use of these new Mycobacterium bovis antigens in the in the differentiation of animals vaccinated against paratuberculosis or infected with tuberculosis by improving the specificity of bovine PPD. In contrast, the two Map antigens tested in this study did not improve the sensitivity of johnin or avian PPD in the detection of vaccinated or Map-infected goats. This research was funded by Ministry of Economy, Industry and Competitiveness through the research project with reference: AGL2012-39818-C02-01 titled “Development of pathogenesis and immunization models in paratuberculosis”. Marcos Royo and Noive Arteche-Villasol were recipients of a predoctoral contract for training of doctors by the Ministry of Economy, Industry and Competitiveness. Peer reviewed Multidisciplinary Digital Publishing Institute http://hdl.handle.net/10261/286986 |
| title |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis |
| spellingShingle |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis Fernández Fernández, Miguel Paratuberculosis Vaccination Ruminants Mycobacterium avium subspecies paratuberculosis Interferon-gamma Immune response Tuberculosis Antigens |
| title_short |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis |
| title_full |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis |
| title_fullStr |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis |
| title_full_unstemmed |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis |
| title_sort |
Peripheral IFN-ɣ production after blood stimulation with different mycobacterial antigens in goats vaccinated against paratuberculosis |
| author |
Fernández Fernández, Miguel |
| author_facet |
Fernández Fernández, Miguel Royo González, Marcos Arteche-Villasol, Noive Ferreras, Mª del Carmen Pérez Pérez, Valentín |
| author_role |
author |
| author2 |
Royo González, Marcos Arteche-Villasol, Noive Ferreras, Mª del Carmen Pérez Pérez, Valentín |
| author2_role |
author author author author |
| topic |
Paratuberculosis Vaccination Ruminants Mycobacterium avium subspecies paratuberculosis Interferon-gamma Immune response Tuberculosis Antigens |
| topic_facet |
Paratuberculosis Vaccination Ruminants Mycobacterium avium subspecies paratuberculosis Interferon-gamma Immune response Tuberculosis Antigens |
| description |
11 páginas, 5 figuras. |
| publishDate |
2022 |
| format |
article |
| status_str |
publishedVersion |
| url |
http://hdl.handle.net/10261/286986 |
| eu_rights_str_mv |
openAccess |
| publisher |
Multidisciplinary Digital Publishing Institute |
| institution |
Consejo Superior de Investigaciones Científicas (CSIC) |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| _version_ |
1878430670370373632 |
| publishDateSort |
2022 |
| author_browse |
Arteche-Villasol, Noive Fernández Fernández, Miguel Ferreras, Mª del Carmen Pérez Pérez, Valentín Royo González, Marcos |
| publisherStr |
Multidisciplinary Digital Publishing Institute |
| score |
6,9303427 |