Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration

The frontiers of bioimaging encompass cutting-edge technologies and methodologies that enhance the visualization, analysis, and interpretation of biological structures and processes with unprecedented precision. In this context, fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerf...

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Authors: Blas Gómez, Sofía, Alonso Moreno, Carlos, Garzón Ruiz, Andrés, Bravo Pérez, Iván
Format: article
Publication Date:2025
Country:España
Institution:Universidad de Castilla-La Mancha
Repository:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/46145
Online Access:https://doi.org/10.1016/j.xinn.2025.101081
https://hdl.handle.net/10578/46145
Access Level:Open access
Keyword:Bioimaging
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oai_identifier_str oai:ruidera.uclm.es:10578/46145
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spelling Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration Blas Gómez, Sofía Alonso Moreno, Carlos Garzón Ruiz, Andrés Bravo Pérez, Iván Bioimaging The frontiers of bioimaging encompass cutting-edge technologies and methodologies that enhance the visualization, analysis, and interpretation of biological structures and processes with unprecedented precision. In this context, fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerful technique that enables quantitative and concentration-independent imaging by measuring the fluorescence decay time of molecular probes. Unlike intensity-based fluorescence methods, FLIM provides robust data on molecular interactions, environments, and biomarker presence, making it invaluable for bioimaging. However, its widespread adoption remains constrained by technical complexity, the high cost of instrumentation, and the underdevelopment of fluorescence lifetime probes with AIE characteristics. A promising solution to these challenges lies in aggregation-induced emission (AIE) fluorophores, which overcome issues such as aggregation-caused quenching and photobleaching. Despite their potential, AIE fluorophores have been rarely integrated with FLIM, leaving a significant gap in bioimaging research. Integrating FLIM with AIE probes could revolutionize bioimaging, enabling precise, stable, and environment-sensitive lifetime imaging for diagnostics, metabolism, and live-cell monitoring. In this perspective, we examine the potential of FLIM-AIE integration in bioimaging, highlighting its advantages, identifying key barriers, and proposing strategies to promote its use in biomedical research. We highlight the critical role of interdisciplinary collaboration, enhanced training programs, and targeted funding initiatives in accelerating innovation in the field of bioimaging. Main text Elsevier https://doi.org/10.1016/j.xinn.2025.101081 https://hdl.handle.net/10578/46145
title Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
spellingShingle Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
Blas Gómez, Sofía
Bioimaging
title_short Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_full Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_fullStr Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_full_unstemmed Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
title_sort Unlocking new frontiers in bioimaging: The power of FLIM-AIE integration
author Blas Gómez, Sofía
author_facet Blas Gómez, Sofía
Alonso Moreno, Carlos
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author_role author
author2 Alonso Moreno, Carlos
Garzón Ruiz, Andrés
Bravo Pérez, Iván
author2_role author
author
author
topic Bioimaging
topic_facet Bioimaging
description The frontiers of bioimaging encompass cutting-edge technologies and methodologies that enhance the visualization, analysis, and interpretation of biological structures and processes with unprecedented precision. In this context, fluorescence lifetime imaging microscopy (FLIM) has emerged as a powerful technique that enables quantitative and concentration-independent imaging by measuring the fluorescence decay time of molecular probes. Unlike intensity-based fluorescence methods, FLIM provides robust data on molecular interactions, environments, and biomarker presence, making it invaluable for bioimaging. However, its widespread adoption remains constrained by technical complexity, the high cost of instrumentation, and the underdevelopment of fluorescence lifetime probes with AIE characteristics. A promising solution to these challenges lies in aggregation-induced emission (AIE) fluorophores, which overcome issues such as aggregation-caused quenching and photobleaching. Despite their potential, AIE fluorophores have been rarely integrated with FLIM, leaving a significant gap in bioimaging research. Integrating FLIM with AIE probes could revolutionize bioimaging, enabling precise, stable, and environment-sensitive lifetime imaging for diagnostics, metabolism, and live-cell monitoring. In this perspective, we examine the potential of FLIM-AIE integration in bioimaging, highlighting its advantages, identifying key barriers, and proposing strategies to promote its use in biomedical research. We highlight the critical role of interdisciplinary collaboration, enhanced training programs, and targeted funding initiatives in accelerating innovation in the field of bioimaging. Main text
publishDate 2025
format article
url https://doi.org/10.1016/j.xinn.2025.101081
https://hdl.handle.net/10578/46145
eu_rights_str_mv openAccess
publisher Elsevier
institution Universidad de Castilla-La Mancha
collection RUIdeRA. Repositorio Institucional de la UCLM
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
instname_str Universidad de Castilla-La Mancha
_version_ 1878432753251254272
publishDateSort 2025
author_browse Alonso Moreno, Carlos
Blas Gómez, Sofía
Bravo Pérez, Iván
Garzón Ruiz, Andrés
publisherStr Elsevier
score 6,9303427

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