Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei

Trypanosoma brucei is the causative agent of human African sleeping sickness and the cattle disease nagana. T. brucei is dependent on glycoproteins for its survival and infectivity throughout its life cycle. Here we report the functional characterization of TbGT3, a glycosyltransferase expressed in...

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Autores: Izquierdo Lázaro, Luis, Acosta-Serrano, Alvaro, Mehlert, Angela, Ferguson, Michael A. J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/99511
Acceso en línea:https://hdl.handle.net/2445/99511
Access Level:acceso abierto
Palabra clave:Malalties parasitàries
Protozoosi
Parasitic diseases
Protozoan diseases
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spelling Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei Izquierdo Lázaro, Luis Acosta-Serrano, Alvaro Mehlert, Angela Ferguson, Michael A. J. Malalties parasitàries Protozoosi Parasitic diseases Protozoan diseases Trypanosoma brucei is the causative agent of human African sleeping sickness and the cattle disease nagana. T. brucei is dependent on glycoproteins for its survival and infectivity throughout its life cycle. Here we report the functional characterization of TbGT3, a glycosyltransferase expressed in the bloodstream and procyclic-form of the parasite. Bloodstream and procyclic-form TbGT3 conditional null mutants were created and both exhibited normal growth under permissive and non-permissive conditions. Under non-permissive conditions, the normal glycosylation of the major glycoprotein of bloodstream form T. brucei, the variant surface glycoprotein, and the absence of major alterations in lectin binding to other glycoproteins suggested that the major function of TbGT3 occurs in the procyclic form of the parasite. Consistent with this, the major surface glycoprotein of the procyclic form, procyclin, exhibited a marked reduction in molecular weight due to changes in glycosylphosphatidylinositol (GPI) anchor side-chains. Structural analysis of the mutant procyclin GPI anchors indicated that TbGT3 encodes a UDP-Gal: beta-GlcNAc-GPI beta1-3 Gal transferase. Despite the alterations in GPI anchor side chains, TbGT3 conditional null mutants remained infectious to tsetse flies under non-permissive conditions. Oxford University Press https://hdl.handle.net/2445/99511
title Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
spellingShingle Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
Izquierdo Lázaro, Luis
Malalties parasitàries
Protozoosi
Parasitic diseases
Protozoan diseases
title_short Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
title_full Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
title_fullStr Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
title_full_unstemmed Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
title_sort Identification of a glycosylphosphatidylinositol anchor-modifying beta1-3 galactosyltransferase in Trypanosoma brucei
author Izquierdo Lázaro, Luis
author_facet Izquierdo Lázaro, Luis
Acosta-Serrano, Alvaro
Mehlert, Angela
Ferguson, Michael A. J.
author_role author
author2 Acosta-Serrano, Alvaro
Mehlert, Angela
Ferguson, Michael A. J.
author2_role author
author
author
topic Malalties parasitàries
Protozoosi
Parasitic diseases
Protozoan diseases
topic_facet Malalties parasitàries
Protozoosi
Parasitic diseases
Protozoan diseases
description Trypanosoma brucei is the causative agent of human African sleeping sickness and the cattle disease nagana. T. brucei is dependent on glycoproteins for its survival and infectivity throughout its life cycle. Here we report the functional characterization of TbGT3, a glycosyltransferase expressed in the bloodstream and procyclic-form of the parasite. Bloodstream and procyclic-form TbGT3 conditional null mutants were created and both exhibited normal growth under permissive and non-permissive conditions. Under non-permissive conditions, the normal glycosylation of the major glycoprotein of bloodstream form T. brucei, the variant surface glycoprotein, and the absence of major alterations in lectin binding to other glycoproteins suggested that the major function of TbGT3 occurs in the procyclic form of the parasite. Consistent with this, the major surface glycoprotein of the procyclic form, procyclin, exhibited a marked reduction in molecular weight due to changes in glycosylphosphatidylinositol (GPI) anchor side-chains. Structural analysis of the mutant procyclin GPI anchors indicated that TbGT3 encodes a UDP-Gal: beta-GlcNAc-GPI beta1-3 Gal transferase. Despite the alterations in GPI anchor side chains, TbGT3 conditional null mutants remained infectious to tsetse flies under non-permissive conditions.
publishDate 2015
format article
status_str publishedVersion
url https://hdl.handle.net/2445/99511
eu_rights_str_mv openAccess
publisher Oxford University Press
institution Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
collection Recercat. Dipósit de la Recerca de Catalunya
reponame_str Recercat. Dipósit de la Recerca de Catalunya
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
_version_ 1878432773690097664
publishDateSort 2015
author_browse Acosta-Serrano, Alvaro
Ferguson, Michael A. J.
Izquierdo Lázaro, Luis
Mehlert, Angela
publisherStr Oxford University Press
score 6,924472

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