Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor
A novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competiti...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/42742 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/42742 |
| Access Level: | acceso abierto |
| Palabra clave: | 543 Adrenocorticotropin Cortisol Electrochemical immunosensor Phenyl boronic acid Química analítica (Química) 2301 Química Analítica |
| id |
ES_3cc9e8307be7fae496dee45daefce18e |
|---|---|
| oai_identifier_str |
oai:docta.ucm.es:20.500.14352/42742 |
| network_acronym_str |
ES |
| network_name_str |
España |
| spelling |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor Moreno Guzmán, María González Cortés, Araceli Yáñez-Sedeño Orive, Paloma 543 Adrenocorticotropin Cortisol Electrochemical immunosensor Phenyl boronic acid Química analítica (Química) 2301 Química Analítica A novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competitive immunoassays involved biotinylated ACTH and alkaline phosphatase labelled streptavidin, or alkaline phosphatase labelled cortisol. Differential pulse voltammetry upon 1-naphtyl phosphate addition was employed to monitor the affinity reactions. The ranges of linearity were 5.0×10−5−0.1 and 0.1−500 ng/mL for ACTH and cortisol. The usefulness of the dual immunosensor was demonstrated by analyzing certified human serum samples with good recoveries. Wiley-VCH https://hdl.handle.net/20.500.14352/42742 |
| title |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor |
| spellingShingle |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor Moreno Guzmán, María 543 Adrenocorticotropin Cortisol Electrochemical immunosensor Phenyl boronic acid Química analítica (Química) 2301 Química Analítica |
| title_short |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor |
| title_full |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor |
| title_fullStr |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor |
| title_full_unstemmed |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor |
| title_sort |
Multiplexed Ultrasensitive Determination of Adrenocorticotropin and Cortisol Hormones at a Dual Electrochemical Immunosensor |
| author |
Moreno Guzmán, María |
| author_facet |
Moreno Guzmán, María González Cortés, Araceli Yáñez-Sedeño Orive, Paloma |
| author_role |
author |
| author2 |
González Cortés, Araceli Yáñez-Sedeño Orive, Paloma |
| author2_role |
author author |
| topic |
543 Adrenocorticotropin Cortisol Electrochemical immunosensor Phenyl boronic acid Química analítica (Química) 2301 Química Analítica |
| topic_facet |
543 Adrenocorticotropin Cortisol Electrochemical immunosensor Phenyl boronic acid Química analítica (Química) 2301 Química Analítica |
| description |
A novel dual electrochemical immunosensor for the multiplexed determination of adrenocorticotropin (ACTH) and cortisol is reported. Aminophenylboronic acid-modified dual screen-printed carbon electrodes were prepared on which the corresponding ACTH and cortisol antibodies were immobilized. Competitive immunoassays involved biotinylated ACTH and alkaline phosphatase labelled streptavidin, or alkaline phosphatase labelled cortisol. Differential pulse voltammetry upon 1-naphtyl phosphate addition was employed to monitor the affinity reactions. The ranges of linearity were 5.0×10−5−0.1 and 0.1−500 ng/mL for ACTH and cortisol. The usefulness of the dual immunosensor was demonstrated by analyzing certified human serum samples with good recoveries. |
| publishDate |
2012 |
| format |
article |
| url |
https://hdl.handle.net/20.500.14352/42742 |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| publisher |
Wiley-VCH |
| institution |
Universidad Complutense de Madrid (UCM) |
| collection |
Docta Complutense |
| reponame_str |
Docta Complutense |
| instname_str |
Universidad Complutense de Madrid (UCM) |
| _version_ |
1878433634071871488 |
| publishDateSort |
2012 |
| author_browse |
González Cortés, Araceli Moreno Guzmán, María Yáñez-Sedeño Orive, Paloma |
| publisherStr |
Wiley-VCH |
| score |
6,9303427 |