Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells
Nanoparticle uptake by cells is a key parameter in their performance in biomedical applications. However, the use of quantitative, non-destructive techniques to obtain the amount of nanoparticles internalized by cells is still uncommon. We have studied the cellular uptake and the toxicity of core-sh...
| Autores: | , , , , , , , , , |
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| Formato: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Recursos: | Universidad Camilo José Cela (UCJC) |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/44857 |
| Acesso em linha: | https://doi.org/10.1098/rsos.231839 https://royalsocietypublishing.org/doi/full/10.1098/rsos.231839 https://hdl.handle.net/10578/44857 |
| Access Level: | acceso abierto |
| Palavra-chave: | cellular uptakena maghemite-silica magnetometry noparticle toxicity |
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oai:ruidera.uclm.es:10578/44857 |
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ES |
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España |
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Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells López Martín, Raúl Aranda Sobrino, Nieves de Enciso Campos, Nerea Sanchez , Elena H Castañeda Peñalvo, Gregorio Lee, Su Seong Binns, Chris Ballesteros Yáñez, Inmaculada Toro Sánchez, José Ángel de Castillo Sarmiento, Carlos Alberto cellular uptakena maghemite-silica magnetometry noparticle toxicity Nanoparticle uptake by cells is a key parameter in their performance in biomedical applications. However, the use of quantitative, non-destructive techniques to obtain the amount of nanoparticles internalized by cells is still uncommon. We have studied the cellular uptake and the toxicity of core-shell maghemite-silica magnetic nanoparticles (MNPs), with a core diameter of 9 nm and a shell thickness of 3 nm. The internalization of the nanoparticles by mouse neuroblastoma 2a cells was evaluated by sensitive and non-destructive Superconducting Quantum Interference Device (SQUID) magnetometry and corroborated by graphite furnace atomic absorption spectroscopy. We were thus able to study the toxicity of the nanoparticles for well-quantified MNP uptake in terms of nanoparticle density within the cell. No significant variation in cell viability or growth rate was detected for any tested exposure. Yet, an increase in both the amount of mitochondrial superoxide and in the lysosomal activity was detected for the highest concentration (100 µg ml-1) and incubation time (24 h), suggesting the onset of a disruption in ROS homeostasis, which may lead to an impairment in antioxidant responses. Our results validate SQUID magnetometry as a sensitive technique to quantify MNP uptake and demonstrate the non-toxic nature of these core-shell MNPs under our culture conditions. ROYAL SOCIETY https://doi.org/10.1098/rsos.231839 https://royalsocietypublishing.org/doi/full/10.1098/rsos.231839 https://hdl.handle.net/10578/44857 |
| title |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells |
| spellingShingle |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells López Martín, Raúl cellular uptakena maghemite-silica magnetometry noparticle toxicity |
| title_short |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells |
| title_full |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells |
| title_fullStr |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells |
| title_full_unstemmed |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells |
| title_sort |
Toxicity and magnetometry evaluation of the uptake of core-shell maghemite-silica nanoparticles by neuroblastoma cells |
| author |
López Martín, Raúl |
| author_facet |
López Martín, Raúl Aranda Sobrino, Nieves de Enciso Campos, Nerea Sanchez , Elena H Castañeda Peñalvo, Gregorio Lee, Su Seong Binns, Chris Ballesteros Yáñez, Inmaculada Toro Sánchez, José Ángel de Castillo Sarmiento, Carlos Alberto |
| author_role |
author |
| author2 |
Aranda Sobrino, Nieves de Enciso Campos, Nerea Sanchez , Elena H Castañeda Peñalvo, Gregorio Lee, Su Seong Binns, Chris Ballesteros Yáñez, Inmaculada Toro Sánchez, José Ángel de Castillo Sarmiento, Carlos Alberto |
| author2_role |
author author author author author author author author author |
| topic |
cellular uptakena maghemite-silica magnetometry noparticle toxicity |
| topic_facet |
cellular uptakena maghemite-silica magnetometry noparticle toxicity |
| description |
Nanoparticle uptake by cells is a key parameter in their performance in biomedical applications. However, the use of quantitative, non-destructive techniques to obtain the amount of nanoparticles internalized by cells is still uncommon. We have studied the cellular uptake and the toxicity of core-shell maghemite-silica magnetic nanoparticles (MNPs), with a core diameter of 9 nm and a shell thickness of 3 nm. The internalization of the nanoparticles by mouse neuroblastoma 2a cells was evaluated by sensitive and non-destructive Superconducting Quantum Interference Device (SQUID) magnetometry and corroborated by graphite furnace atomic absorption spectroscopy. We were thus able to study the toxicity of the nanoparticles for well-quantified MNP uptake in terms of nanoparticle density within the cell. No significant variation in cell viability or growth rate was detected for any tested exposure. Yet, an increase in both the amount of mitochondrial superoxide and in the lysosomal activity was detected for the highest concentration (100 µg ml-1) and incubation time (24 h), suggesting the onset of a disruption in ROS homeostasis, which may lead to an impairment in antioxidant responses. Our results validate SQUID magnetometry as a sensitive technique to quantify MNP uptake and demonstrate the non-toxic nature of these core-shell MNPs under our culture conditions. |
| publishDate |
2024 |
| format |
article |
| url |
https://doi.org/10.1098/rsos.231839 https://royalsocietypublishing.org/doi/full/10.1098/rsos.231839 https://hdl.handle.net/10578/44857 |
| eu_rights_str_mv |
openAccess |
| publisher |
ROYAL SOCIETY |
| institution |
Universidad Camilo José Cela (UCJC) |
| collection |
RUIdeRA. Repositorio Institucional de la UCLM |
| reponame_str |
RUIdeRA. Repositorio Institucional de la UCLM |
| instname_str |
Universidad Camilo José Cela (UCJC) |
| _version_ |
1878434200076419072 |
| publishDateSort |
2024 |
| author_browse |
Aranda Sobrino, Nieves Ballesteros Yáñez, Inmaculada Binns, Chris Castañeda Peñalvo, Gregorio Castillo Sarmiento, Carlos Alberto Lee, Su Seong López Martín, Raúl Sanchez , Elena H Toro Sánchez, José Ángel de de Enciso Campos, Nerea |
| publisherStr |
ROYAL SOCIETY |
| score |
6,9303427 |