The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease
Despite conjugate vaccine introduction to prevent invasive pneumococcal disease (IPD), serotype replacement by non-vaccine serotypes is a constant concern. In this study, we elucidate the rise of serotype 8 causing IPD in Spain. We evaluated isolates received during the period 2008-2023 including wh...
| Autores: | , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/222835 |
| Acceso en línea: | https://hdl.handle.net/2445/222835 |
| Access Level: | acceso abierto |
| Palabra clave: | Infeccions per pneumococs Vacuna antipneumocòccica Pneumococcal Infections Pneumococcal vaccine |
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oai:recercat.cat:2445/222835 |
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España |
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The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease Pérez García, Covadonga González Díaz, Aida Domenech, Mirian Llamosí, Mirella Úbeda, Aída Sanz, Juan Carlos García, Ernesto Ardanuy Tisaire, María Carmen Sempere, Julio Yuste, José Enrique Infeccions per pneumococs Vacuna antipneumocòccica Pneumococcal Infections Pneumococcal vaccine Despite conjugate vaccine introduction to prevent invasive pneumococcal disease (IPD), serotype replacement by non-vaccine serotypes is a constant concern. In this study, we elucidate the rise of serotype 8 causing IPD in Spain. We evaluated isolates received during the period 2008-2023 including whole genome sequencing characterization and host-pathogen interaction studies. Serotype 8 has emerged as one of the most prevalent serotypes causing IPD in both children and adults. CC53/GPSC3 carrying pspC 6.11 was the dominant lineage in recent years, displaying increased adhesion to lung cells, enhanced biofilm formation, higher factor H recruitment, improved phagocytosis evasion, and greater virulence in a mice pneumonia model than other co-circulating lineages which could explain its predominance. Morphologically, serotype 8 strains exhibit two appearances on blood agar plates: mucoid colonies, and non-mucoid variants. Molecular characterization revealed that non-mucoid variants harbour mutations in the wchA gene and/or others within the capsular operon, leading to increased adhesion and biofilm formation, albeit with reduced immune evasion capacity. Serotype 8 has become a major cause of IPD, with CC53/GPSC3 as the dominant lineage due to its pathogenic advantages. The versatility of the capsular operon contributes to its success in causing IPD. The use of vaccines with broader coverage, such as PCV20 or PCV21, containing this serotype, may offer an effective strategy to ameliorate the impact on IPD by serotype 8. Informa UK Limited https://hdl.handle.net/2445/222835 |
| title |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease |
| spellingShingle |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease Pérez García, Covadonga Infeccions per pneumococs Vacuna antipneumocòccica Pneumococcal Infections Pneumococcal vaccine |
| title_short |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease |
| title_full |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease |
| title_fullStr |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease |
| title_full_unstemmed |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease |
| title_sort |
The rise of serotype 8 is associated with lineages and mutations in the capsular operon with different potential to produce invasive pneumococcal disease |
| author |
Pérez García, Covadonga |
| author_facet |
Pérez García, Covadonga González Díaz, Aida Domenech, Mirian Llamosí, Mirella Úbeda, Aída Sanz, Juan Carlos García, Ernesto Ardanuy Tisaire, María Carmen Sempere, Julio Yuste, José Enrique |
| author_role |
author |
| author2 |
González Díaz, Aida Domenech, Mirian Llamosí, Mirella Úbeda, Aída Sanz, Juan Carlos García, Ernesto Ardanuy Tisaire, María Carmen Sempere, Julio Yuste, José Enrique |
| author2_role |
author author author author author author author author author |
| topic |
Infeccions per pneumococs Vacuna antipneumocòccica Pneumococcal Infections Pneumococcal vaccine |
| topic_facet |
Infeccions per pneumococs Vacuna antipneumocòccica Pneumococcal Infections Pneumococcal vaccine |
| description |
Despite conjugate vaccine introduction to prevent invasive pneumococcal disease (IPD), serotype replacement by non-vaccine serotypes is a constant concern. In this study, we elucidate the rise of serotype 8 causing IPD in Spain. We evaluated isolates received during the period 2008-2023 including whole genome sequencing characterization and host-pathogen interaction studies. Serotype 8 has emerged as one of the most prevalent serotypes causing IPD in both children and adults. CC53/GPSC3 carrying pspC 6.11 was the dominant lineage in recent years, displaying increased adhesion to lung cells, enhanced biofilm formation, higher factor H recruitment, improved phagocytosis evasion, and greater virulence in a mice pneumonia model than other co-circulating lineages which could explain its predominance. Morphologically, serotype 8 strains exhibit two appearances on blood agar plates: mucoid colonies, and non-mucoid variants. Molecular characterization revealed that non-mucoid variants harbour mutations in the wchA gene and/or others within the capsular operon, leading to increased adhesion and biofilm formation, albeit with reduced immune evasion capacity. Serotype 8 has become a major cause of IPD, with CC53/GPSC3 as the dominant lineage due to its pathogenic advantages. The versatility of the capsular operon contributes to its success in causing IPD. The use of vaccines with broader coverage, such as PCV20 or PCV21, containing this serotype, may offer an effective strategy to ameliorate the impact on IPD by serotype 8. |
| publishDate |
2025 |
| format |
article |
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publishedVersion |
| url |
https://hdl.handle.net/2445/222835 |
| eu_rights_str_mv |
openAccess |
| publisher |
Informa UK Limited |
| institution |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| collection |
Recercat. Dipósit de la Recerca de Catalunya |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| _version_ |
1878434218146529280 |
| publishDateSort |
2025 |
| author_browse |
Ardanuy Tisaire, María Carmen Domenech, Mirian García, Ernesto González Díaz, Aida Llamosí, Mirella Pérez García, Covadonga Sanz, Juan Carlos Sempere, Julio Yuste, José Enrique Úbeda, Aída |
| publisherStr |
Informa UK Limited |
| score |
6.9303427 |