An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol
Reduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2015 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/124311 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/124311 |
| Access Level: | acceso abierto |
| Palabra clave: | 543 Graphene Adiponectin Electrochemical immunosensor Carboxymethyl cellulose Mix&Go Human serum Química 2301 Química Analítica |
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oai:docta.ucm.es:20.500.14352/124311 |
| network_acronym_str |
ES |
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España |
| spelling |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol Arenas Sevillano, Cristian Brenabé Sánchez Tirado, Esther Ojeda Fernández, Irene Gomez-Suarez, C.A. González Cortés, Araceli Villalonga Santana, Reynaldo Yáñez-Sedeño Orive, Paloma Pingarrón Carrazón, José Manuel 543 Graphene Adiponectin Electrochemical immunosensor Carboxymethyl cellulose Mix&Go Human serum Química 2301 Química Analítica Reduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction with sodium borohydride. A sandwich-type immunoassay was employed involving the commercial metal-complexes based polymer Mix & Go™ for the stable and oriented immobilization of anti-APN capture antibody. Biotinylated-anti-APN and HRP-Strept were used for the assay configuration. The APN quantification was performed by amperometry at −200 mV using the hydrogen peroxide/hydroquinone enzyme substrate/mediator system. A sigmoidal calibration plot for APN in the 0.1–50 μg/mL range, with a linear portion in the 0.5–10.0 μg/mL APN concentration range was obtained. The calculated limit of detection (3sb/m) was 61 ng/mL APN. The usefulness of the immunosensor was evaluated by analyzing human serum from hypercholesterolemia or diabetes patients. Elsevier https://hdl.handle.net/20.500.14352/124311 |
| title |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol |
| spellingShingle |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol Arenas Sevillano, Cristian Brenabé 543 Graphene Adiponectin Electrochemical immunosensor Carboxymethyl cellulose Mix&Go Human serum Química 2301 Química Analítica |
| title_short |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol |
| title_full |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol |
| title_fullStr |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol |
| title_full_unstemmed |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol |
| title_sort |
An electrochemical immunosensor for adiponectin using reduced graphene oxide–carboxymethylcellulose hybrid as electrode scaffol |
| author |
Arenas Sevillano, Cristian Brenabé |
| author_facet |
Arenas Sevillano, Cristian Brenabé Sánchez Tirado, Esther Ojeda Fernández, Irene Gomez-Suarez, C.A. González Cortés, Araceli Villalonga Santana, Reynaldo Yáñez-Sedeño Orive, Paloma Pingarrón Carrazón, José Manuel |
| author_role |
author |
| author2 |
Sánchez Tirado, Esther Ojeda Fernández, Irene Gomez-Suarez, C.A. González Cortés, Araceli Villalonga Santana, Reynaldo Yáñez-Sedeño Orive, Paloma Pingarrón Carrazón, José Manuel |
| author2_role |
author author author author author author author |
| topic |
543 Graphene Adiponectin Electrochemical immunosensor Carboxymethyl cellulose Mix&Go Human serum Química 2301 Química Analítica |
| topic_facet |
543 Graphene Adiponectin Electrochemical immunosensor Carboxymethyl cellulose Mix&Go Human serum Química 2301 Química Analítica |
| description |
Reduced graphene oxide–carboxymethylcellulose hybrid (CMC–rGO) was used for the development of a novel electrochemical immunosensor for the determination of adiponectin (APN) cytokine. The hybrid material was synthesized by covalent binding of oxidized CMC to GO layers followed by chemical reduction with sodium borohydride. A sandwich-type immunoassay was employed involving the commercial metal-complexes based polymer Mix & Go™ for the stable and oriented immobilization of anti-APN capture antibody. Biotinylated-anti-APN and HRP-Strept were used for the assay configuration. The APN quantification was performed by amperometry at −200 mV using the hydrogen peroxide/hydroquinone enzyme substrate/mediator system. A sigmoidal calibration plot for APN in the 0.1–50 μg/mL range, with a linear portion in the 0.5–10.0 μg/mL APN concentration range was obtained. The calculated limit of detection (3sb/m) was 61 ng/mL APN. The usefulness of the immunosensor was evaluated by analyzing human serum from hypercholesterolemia or diabetes patients. |
| publishDate |
2015 |
| format |
article |
| url |
https://hdl.handle.net/20.500.14352/124311 |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| publisher |
Elsevier |
| institution |
Universidad Complutense de Madrid (UCM) |
| collection |
Docta Complutense |
| reponame_str |
Docta Complutense |
| instname_str |
Universidad Complutense de Madrid (UCM) |
| _version_ |
1878434586597261312 |
| publishDateSort |
2015 |
| author_browse |
Arenas Sevillano, Cristian Brenabé Gomez-Suarez, C.A. González Cortés, Araceli Ojeda Fernández, Irene Pingarrón Carrazón, José Manuel Sánchez Tirado, Esther Villalonga Santana, Reynaldo Yáñez-Sedeño Orive, Paloma |
| publisherStr |
Elsevier |
| score |
6,9303427 |