Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources
[EN] BackgroundForest species ranges are confined by environmental limitations such as cold stress. The natural range shifts of pine forests due to climate change and proactive-assisted population migration may each be constrained by the ability of pine species to tolerate low temperatures, especial...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/142522 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/142522 |
| Access Level: | acceso abierto |
| Palabra clave: | Pinus halepensis Climate change Cold stress Soluble sugars Osmolytes Antioxidants Glutathione Free amino acids Seed source evaluation BIOQUIMICA Y BIOLOGIA MOLECULAR TECNOLOGIA DEL MEDIO AMBIENTE |
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oai:riunet.upv.es:10251/142522 |
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ES |
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España |
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Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources Taïbi, Khaled Vilagrosa, Alberto López-Nicolás, José M. Campo García, Antonio Dámaso Del|||0000-0002-5279-4215 Belles Albert, José Mª|||0000-0003-0362-9616 López-Gresa, María Pilar|||0000-0001-9251-0160 Mulet, José Miguel|||0000-0002-9087-3838 Pinus halepensis Climate change Cold stress Soluble sugars Osmolytes Antioxidants Glutathione Free amino acids Seed source evaluation BIOQUIMICA Y BIOLOGIA MOLECULAR TECNOLOGIA DEL MEDIO AMBIENTE [EN] BackgroundForest species ranges are confined by environmental limitations such as cold stress. The natural range shifts of pine forests due to climate change and proactive-assisted population migration may each be constrained by the ability of pine species to tolerate low temperatures, especially in northern latitudes or in high altitudes. The aim of this study is to characterize the response of cold-tolerant versus cold-sensitive Pinus halepensis (P. halepensis) seedlings at the physiological and the molecular level under controlled cold conditions to identify distinctive features which allow us to explain the phenotypic difference. With this objective gas-exchange and water potential was determined and the photosynthetic pigments, soluble sugars, glutathione and free amino acids content were measured in seedlings of different provenances under control and cold stress conditions.ResultsGlucose and fructose content can be highlighted as a potential distinctive trait for cold-tolerant P. halepensis seedlings. At the amino acid level, there was a significant increase and accumulation of glutathione, proline, glutamic acid, histidine, arginine and tryptophan along with a significant decrease of glycine.ConclusionOur results established that the main difference between cold-tolerant and cold-sensitive seedlings of P. halepensis is the ability to accumulate the antioxidant glutathione and osmolytes such as glucose and fructose, proline and arginine. This study is a part of the research project: "Application of molecular biology techniques in forest restoration in Mediterranean environments, PAID-05-11" funded by the Universitat Politecnica de Valencia (UPV), program for supporting R&D of new multidisciplinary research lines. The authors are grateful to the Ministerio de Economia y Competitividad AGL2014-57431-P and BIO2016-77776-P. AV was supported by project Survive-2 (CGL2015-69773-C2-2-P MINECO/FEDER) by the Spanish Government and Prometeo program (DESESTRES Generalitat Valenciana). CEAM is funded by Generalitat Valenciana. None of the funding bodies was involved in the design of the study; collection, analysis, and interpretation of data; and in writing of the manuscript which were performed entirely by the signing authors. Springer (Biomed Central Ltd.) https://riunet.upv.es/handle/10251/142522 |
| title |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources |
| spellingShingle |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources Taïbi, Khaled Pinus halepensis Climate change Cold stress Soluble sugars Osmolytes Antioxidants Glutathione Free amino acids Seed source evaluation BIOQUIMICA Y BIOLOGIA MOLECULAR TECNOLOGIA DEL MEDIO AMBIENTE |
| title_short |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources |
| title_full |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources |
| title_fullStr |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources |
| title_full_unstemmed |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources |
| title_sort |
Distinctive physiological and molecular responses to cold stress among cold-tolerant and cold-sensitive Pinus halepensis seed sources |
| author |
Taïbi, Khaled |
| author_facet |
Taïbi, Khaled Vilagrosa, Alberto López-Nicolás, José M. Campo García, Antonio Dámaso Del|||0000-0002-5279-4215 Belles Albert, José Mª|||0000-0003-0362-9616 López-Gresa, María Pilar|||0000-0001-9251-0160 Mulet, José Miguel|||0000-0002-9087-3838 |
| author_role |
author |
| author2 |
Vilagrosa, Alberto López-Nicolás, José M. Campo García, Antonio Dámaso Del|||0000-0002-5279-4215 Belles Albert, José Mª|||0000-0003-0362-9616 López-Gresa, María Pilar|||0000-0001-9251-0160 Mulet, José Miguel|||0000-0002-9087-3838 |
| author2_role |
author author author author author author |
| topic |
Pinus halepensis Climate change Cold stress Soluble sugars Osmolytes Antioxidants Glutathione Free amino acids Seed source evaluation BIOQUIMICA Y BIOLOGIA MOLECULAR TECNOLOGIA DEL MEDIO AMBIENTE |
| topic_facet |
Pinus halepensis Climate change Cold stress Soluble sugars Osmolytes Antioxidants Glutathione Free amino acids Seed source evaluation BIOQUIMICA Y BIOLOGIA MOLECULAR TECNOLOGIA DEL MEDIO AMBIENTE |
| description |
[EN] BackgroundForest species ranges are confined by environmental limitations such as cold stress. The natural range shifts of pine forests due to climate change and proactive-assisted population migration may each be constrained by the ability of pine species to tolerate low temperatures, especially in northern latitudes or in high altitudes. The aim of this study is to characterize the response of cold-tolerant versus cold-sensitive Pinus halepensis (P. halepensis) seedlings at the physiological and the molecular level under controlled cold conditions to identify distinctive features which allow us to explain the phenotypic difference. With this objective gas-exchange and water potential was determined and the photosynthetic pigments, soluble sugars, glutathione and free amino acids content were measured in seedlings of different provenances under control and cold stress conditions.ResultsGlucose and fructose content can be highlighted as a potential distinctive trait for cold-tolerant P. halepensis seedlings. At the amino acid level, there was a significant increase and accumulation of glutathione, proline, glutamic acid, histidine, arginine and tryptophan along with a significant decrease of glycine.ConclusionOur results established that the main difference between cold-tolerant and cold-sensitive seedlings of P. halepensis is the ability to accumulate the antioxidant glutathione and osmolytes such as glucose and fructose, proline and arginine. |
| publishDate |
2018 |
| format |
article |
| url |
https://riunet.upv.es/handle/10251/142522 |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| publisher |
Springer (Biomed Central Ltd.) |
| institution |
Universitat Politècnica de València (UPV) |
| collection |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| reponame_str |
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| instname_str |
Universitat Politècnica de València (UPV) |
| _version_ |
1878435366283771904 |
| publishDateSort |
2018 |
| author_browse |
Belles Albert, José Mª|||0000-0003-0362-9616 Campo García, Antonio Dámaso Del|||0000-0002-5279-4215 López-Gresa, María Pilar|||0000-0001-9251-0160 López-Nicolás, José M. Mulet, José Miguel|||0000-0002-9087-3838 Taïbi, Khaled Vilagrosa, Alberto |
| publisherStr |
Springer (Biomed Central Ltd.) |
| score |
6,924472 |