Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters
β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effect...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/109346 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/109346 |
| Access Level: | acceso abierto |
| Palabra clave: | 636.082 Acrosome reaction Capacitation NGF Rabbit sperm Sperm motility Producción animal 3104.11 Reproducción |
| id |
ES_70b9adecd7ca026c4b702a0fa49fa8a5 |
|---|---|
| oai_identifier_str |
oai:docta.ucm.es:20.500.14352/109346 |
| network_acronym_str |
ES |
| network_name_str |
España |
| spelling |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters Gimeno Martos, Silvia Bosa, Luigia Lorenzo González, Pedro Luis Arias Álvarez, María Castellini, Cesare García Rebollar, Pilar García García, Rosa María 636.082 Acrosome reaction Capacitation NGF Rabbit sperm Sperm motility Producción animal 3104.11 Reproducción β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination. Wiley https://hdl.handle.net/20.500.14352/109346 |
| title |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters |
| spellingShingle |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters Gimeno Martos, Silvia 636.082 Acrosome reaction Capacitation NGF Rabbit sperm Sperm motility Producción animal 3104.11 Reproducción |
| title_short |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters |
| title_full |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters |
| title_fullStr |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters |
| title_full_unstemmed |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters |
| title_sort |
Influence of free and microencapsulated recombinant rabbit nerve growth factor with chitosan on rabbit sperm quality parameters |
| author |
Gimeno Martos, Silvia |
| author_facet |
Gimeno Martos, Silvia Bosa, Luigia Lorenzo González, Pedro Luis Arias Álvarez, María Castellini, Cesare García Rebollar, Pilar García García, Rosa María |
| author_role |
author |
| author2 |
Bosa, Luigia Lorenzo González, Pedro Luis Arias Álvarez, María Castellini, Cesare García Rebollar, Pilar García García, Rosa María |
| author2_role |
author author author author author author |
| topic |
636.082 Acrosome reaction Capacitation NGF Rabbit sperm Sperm motility Producción animal 3104.11 Reproducción |
| topic_facet |
636.082 Acrosome reaction Capacitation NGF Rabbit sperm Sperm motility Producción animal 3104.11 Reproducción |
| description |
β-nerve growth factor (βNGF) plays a crucial role in reproductive physiology and sperm quality. Enzymatic activity of seminal plasma and vaginal fluids reduces available βNGF and it has been demonstrated that chitosan microspheres could protect rrβNGF from degradation. This study examined the effects of microencapsulated rrbNGF with chitosan on rabbit sperm viability, motility and capacitation status. Results showed that 0.5 and 1 μg/mL of microencapsulated rrβNGF, as well as free rrβNGF or empty microspheres, did not adversely affect sperm viability or total motility after 2 h of incubation. However, the highest progressivity kinetic parameters were observed with 1 μg/mL free rrβNGF, while the highest curvilinear velocity (VCL) occurred with 0.5 μg/mL microencapsulated rrβNGF. Empty chitosan microspheres did not induce acrosome reaction (AR), but both concentrations of free and rrβNGFch favoured AR during in vitro incubation. The study suggests that using chitosan spheres did not show any adverse effects on sperm traits, unlike free rβNGF and rrβNGFch promoted capacitation and AR. Further research is needed to explore the potential of rrβNGFch in modifying in vitro capacitation and inducing ovulation during artificial insemination. |
| publishDate |
2024 |
| format |
article |
| url |
https://hdl.handle.net/20.500.14352/109346 |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| publisher |
Wiley |
| institution |
Universidad Complutense de Madrid (UCM) |
| collection |
Docta Complutense |
| reponame_str |
Docta Complutense |
| instname_str |
Universidad Complutense de Madrid (UCM) |
| _version_ |
1878436237454344192 |
| publishDateSort |
2024 |
| author_browse |
Arias Álvarez, María Bosa, Luigia Castellini, Cesare García García, Rosa María García Rebollar, Pilar Gimeno Martos, Silvia Lorenzo González, Pedro Luis |
| publisherStr |
Wiley |
| score |
6,924472 |