Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors
22 Pág.
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/334954 |
| Acceso en línea: | http://hdl.handle.net/10261/334954 https://api.elsevier.com/content/abstract/scopus_id/85165642578 |
| Access Level: | acceso abierto |
| Palabra clave: | Molecular biology Omics Transcriptomics |
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Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors Navarrete-López, Paula Maroto, María Pericuesta Camacho, Eva Fernández González, Raúl Lombó, Marta Ramos Ibeas, Priscila Gutiérrez-Adán, Alfonso Molecular biology Omics Transcriptomics 22 Pág. Karyopherins mediate the movement between the nucleus and cytoplasm of specific proteins in diverse cellular processes. Through a loss-of-function approach, we here examine the role of Karyopherin Subunit Alpha 2 (Kpna2) in spermatogenesis. Knockout male mice exhibited reduced body size and sperm motility, increased sperm abnormalities, and led to the dysregulation of testis gene expression and ultimately to infertility. Impaired mRNA expression mainly affected clusters of genes expressed in spermatids and spermatocytes. Downregulated genes included a set of genes that participate in cell adhesion and extracellular matrix (ECM) organization. We detected both the enrichment of some transcription factors that bind to regions around transcription start sites of downregulated genes and the impaired transport of specific factors to the nucleus of spermatid cells. We propose that Kpna2 is essential in the seminiferous tubules for promoting the translocation of testis-specific transcription factors that control the expression of genes related to ECM organization. This research was supported by Grants RTI2018-093548-B-I00 and PID2021-122507OB-I00 funded by MCIN/AEI/10.13039/501100011033/and European Union “NextGenerationEU”/PRTR). P.N was supported by a pre-doctoral fellowship from the Spanish Ministry of Science and Innovation (MICINN) (PRE2019-088813), and M.L was supported by a Juan de la Cierva postdoctoral contract (FJC2019-040385-I) from the MICINN. We gratefully thank CESGA (Supercomputing Center of Galicia) for providing the necessary resources for the data processing. Peer reviewed Elsevier http://hdl.handle.net/10261/334954 https://api.elsevier.com/content/abstract/scopus_id/85165642578 |
| title |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors |
| spellingShingle |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors Navarrete-López, Paula Molecular biology Omics Transcriptomics |
| title_short |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors |
| title_full |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors |
| title_fullStr |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors |
| title_full_unstemmed |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors |
| title_sort |
Loss of the importin Kpna2 causes infertility in male mice by disrupting the translocation of testis-specific transcription factors |
| author |
Navarrete-López, Paula |
| author_facet |
Navarrete-López, Paula Maroto, María Pericuesta Camacho, Eva Fernández González, Raúl Lombó, Marta Ramos Ibeas, Priscila Gutiérrez-Adán, Alfonso |
| author_role |
author |
| author2 |
Maroto, María Pericuesta Camacho, Eva Fernández González, Raúl Lombó, Marta Ramos Ibeas, Priscila Gutiérrez-Adán, Alfonso |
| author2_role |
author author author author author author |
| topic |
Molecular biology Omics Transcriptomics |
| topic_facet |
Molecular biology Omics Transcriptomics |
| description |
22 Pág. |
| publishDate |
2023 |
| format |
article |
| status_str |
publishedVersion |
| url |
http://hdl.handle.net/10261/334954 https://api.elsevier.com/content/abstract/scopus_id/85165642578 |
| eu_rights_str_mv |
openAccess |
| publisher |
Elsevier |
| institution |
Consejo Superior de Investigaciones Científicas (CSIC) |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| _version_ |
1878436537607127040 |
| publishDateSort |
2023 |
| author_browse |
Fernández González, Raúl Gutiérrez-Adán, Alfonso Lombó, Marta Maroto, María Navarrete-López, Paula Pericuesta Camacho, Eva Ramos Ibeas, Priscila |
| publisherStr |
Elsevier |
| score |
6,924472 |