Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,

Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N’,...

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Autores: Voitechovič, E., Bratov, Andrey, Abramova, Natalia, Razumienė, J., Kirsanov, D., Legin, Andrey, Lakshmi, D., Piletsky, S., Whitcombe, M., Ivanova-Mitseva, P. K.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/164221
Acceso en línea:http://hdl.handle.net/10261/164221
Access Level:acceso abierto
Palabra clave:Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
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spelling Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications, Voitechovič, E. Bratov, Andrey Abramova, Natalia Razumienė, J. Kirsanov, D. Legin, Andrey Lakshmi, D. Piletsky, S. Whitcombe, M. Ivanova-Mitseva, P. K. Interdigitated electrode array DET PQQ dependent enzyme Polyaniline Biosensors Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N’,N’-diethyldithiocarbamoylethylamidoethyl) aniline (AnD) was deposited onto 3D-IDEA by chemical polymerisation. It was found that the polymer film resistance depends on the redox-potential of the solution. For the first time polyAnD was used as enzyme immobilisation matrix. Pyrroloquinolinequinone (PQQ) dependent alcohol and glucose dehydrogenases were immobilized on 3D-IDEA covered with polyAnD by two different methods. 3D-IDEA sensors with enzymes, which were immobilised by physisorption on polyAnD layer, showed specific response in the presence of 1 μM of the corresponding substrates. Obtained results revealed that PQQ dependent dehydrogenases can re-oxidize on polyAnD via direct electron transfer (DET) from enzyme active site to the polymer surface. This process can be monitored by methods of electrochemical impedance spectroscopy (EIS) and chronoamperometry. Presented study shows that EIS method gives a useful tool for research of re-oxidation process and interaction of electroactive enzymes with conducting materials giving information required to construct and develop analytical devices. N. Abramova and A. Bratov acknowledge financial support from Spanish Ministry of Science and Innovation (projects AGL2008-05578-C05-05/01; IPT-2011-1055-900000 and CTQ2011-29163-C03-02). E. Voitechovic would like to acknowledge the financial support from St. Petersburg State University PostDoc Grant #12.50.1191.2014 and PhD Student Research Traineeship from the Lithuanian Science Council. Peer reviewed Elsevier http://hdl.handle.net/10261/164221
title Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
spellingShingle Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
Voitechovič, E.
Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
title_short Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_full Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_fullStr Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_full_unstemmed Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
title_sort Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications,
author Voitechovič, E.
author_facet Voitechovič, E.
Bratov, Andrey
Abramova, Natalia
Razumienė, J.
Kirsanov, D.
Legin, Andrey
Lakshmi, D.
Piletsky, S.
Whitcombe, M.
Ivanova-Mitseva, P. K.
author_role author
author2 Bratov, Andrey
Abramova, Natalia
Razumienė, J.
Kirsanov, D.
Legin, Andrey
Lakshmi, D.
Piletsky, S.
Whitcombe, M.
Ivanova-Mitseva, P. K.
author2_role author
author
author
author
author
author
author
author
author
topic Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
topic_facet Interdigitated electrode array
DET
PQQ dependent enzyme
Polyaniline
Biosensors
description Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N’,N’-diethyldithiocarbamoylethylamidoethyl) aniline (AnD) was deposited onto 3D-IDEA by chemical polymerisation. It was found that the polymer film resistance depends on the redox-potential of the solution. For the first time polyAnD was used as enzyme immobilisation matrix. Pyrroloquinolinequinone (PQQ) dependent alcohol and glucose dehydrogenases were immobilized on 3D-IDEA covered with polyAnD by two different methods. 3D-IDEA sensors with enzymes, which were immobilised by physisorption on polyAnD layer, showed specific response in the presence of 1 μM of the corresponding substrates. Obtained results revealed that PQQ dependent dehydrogenases can re-oxidize on polyAnD via direct electron transfer (DET) from enzyme active site to the polymer surface. This process can be monitored by methods of electrochemical impedance spectroscopy (EIS) and chronoamperometry. Presented study shows that EIS method gives a useful tool for research of re-oxidation process and interaction of electroactive enzymes with conducting materials giving information required to construct and develop analytical devices.
publishDate 2015
format article
status_str acceptedVersion
url http://hdl.handle.net/10261/164221
eu_rights_str_mv openAccess
publisher Elsevier
institution Consejo Superior de Investigaciones Científicas (CSIC)
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
_version_ 1878436558718107648
publishDateSort 2015
author_browse Abramova, Natalia
Bratov, Andrey
Ivanova-Mitseva, P. K.
Kirsanov, D.
Lakshmi, D.
Legin, Andrey
Piletsky, S.
Razumienė, J.
Voitechovič, E.
Whitcombe, M.
publisherStr Elsevier
score 6,9303427

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