Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles

Olfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigat...

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Autores: Terni, Beatrice, Pacciolla, Paolo, Masanas, Helena, Gorostiza Langa, Pablo Ignacio, Llobet Berenguer, Artur, 1972-
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/147225
Acceso en línea:https://hdl.handle.net/2445/147225
Access Level:acceso abierto
Palabra clave:Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
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spelling Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles Terni, Beatrice Pacciolla, Paolo Masanas, Helena Gorostiza Langa, Pablo Ignacio Llobet Berenguer, Artur, 1972- Sinapsi Olfacte Fisiologia Metabolisme Synapses Smell Physiology Metabolism Olfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigate rewiring of glomerular connectivity, we show that novel OSN synapses can transfer information immediately after formation, mediating olfactory-guided behavior. Tadpoles recover the ability to detect amino acids 4 days after bilateral olfactory nerve transection. Restoration of olfactory-guided behavior depends on the efficient reinsertion of OSNs to the olfactory bulb. Presynaptic terminals of incipient synaptic contacts generate calcium transients in response to odors, triggering long lasting depolarization of olfactory glomeruli. The functionality of reconnected terminals relies on well-defined readily releasable and cytoplasmic vesicle pools. The continuous growth of non-compartmentalized axonal processes provides a vesicle reservoir to nascent release sites, which contrasts to the gradual development of cytoplasmic vesicle pools in conventional excitatory synapses. The immediate availability of fully functional synapses upon formation supports an age-independent contribution of OSNs to the generation of odor maps. Wiley https://hdl.handle.net/2445/147225
title Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
spellingShingle Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
Terni, Beatrice
Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
title_short Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_full Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_fullStr Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_full_unstemmed Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
title_sort Tight temporal coupling between synaptic rewiring of olfactory glomeruli and the emergence of odor-guided behavior in Xenopus tadpoles
author Terni, Beatrice
author_facet Terni, Beatrice
Pacciolla, Paolo
Masanas, Helena
Gorostiza Langa, Pablo Ignacio
Llobet Berenguer, Artur, 1972-
author_role author
author2 Pacciolla, Paolo
Masanas, Helena
Gorostiza Langa, Pablo Ignacio
Llobet Berenguer, Artur, 1972-
author2_role author
author
author
author
topic Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
topic_facet Sinapsi
Olfacte
Fisiologia
Metabolisme
Synapses
Smell
Physiology
Metabolism
description Olfactory sensory neurons (OSNs) are chemoreceptors that establish excitatory synapses within glomeruli of the olfactory bulb. OSNs undergo continuous turnover throughout life, causing the constant replacement of their synaptic contacts. Using Xenopus tadpoles as an experimental system to investigate rewiring of glomerular connectivity, we show that novel OSN synapses can transfer information immediately after formation, mediating olfactory-guided behavior. Tadpoles recover the ability to detect amino acids 4 days after bilateral olfactory nerve transection. Restoration of olfactory-guided behavior depends on the efficient reinsertion of OSNs to the olfactory bulb. Presynaptic terminals of incipient synaptic contacts generate calcium transients in response to odors, triggering long lasting depolarization of olfactory glomeruli. The functionality of reconnected terminals relies on well-defined readily releasable and cytoplasmic vesicle pools. The continuous growth of non-compartmentalized axonal processes provides a vesicle reservoir to nascent release sites, which contrasts to the gradual development of cytoplasmic vesicle pools in conventional excitatory synapses. The immediate availability of fully functional synapses upon formation supports an age-independent contribution of OSNs to the generation of odor maps.
publishDate 2017
format article
status_str acceptedVersion
url https://hdl.handle.net/2445/147225
eu_rights_str_mv openAccess
publisher Wiley
institution Universidad de Barcelona
collection Dipòsit Digital de la UB
reponame_str Dipòsit Digital de la UB
instname_str Universidad de Barcelona
_version_ 1878735239421886464
publishDateSort 2017
author_browse Gorostiza Langa, Pablo Ignacio
Llobet Berenguer, Artur, 1972-
Masanas, Helena
Pacciolla, Paolo
Terni, Beatrice
publisherStr Wiley
score 6,8972664

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