Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
[EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Tran...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/17970 |
| Acceso en línea: | https://academic.oup.com/nar/article/41/3/1767/2902433 https://hdl.handle.net/10612/17970 |
| Access Level: | acceso abierto |
| Palabra clave: | Biología Phosphate regulation Nitrogen regulation Streptomyces coelicolor PhoR-PhoP GlnR PHO boxes EMSA DNase footprinting 2415.01 Biología Molecular de Microorganismos 2414.02 Fisiología Bacteriana |
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oai:buleria.unileon.es:10612/17970 |
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ES |
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España |
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Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor Sola Landa, Alberto Rodríguez García, Antonio Amin, Rafat Wohlleben, Wolfgang Martín Martín, Juan Francisco Biología Phosphate regulation Nitrogen regulation Streptomyces coelicolor PhoR-PhoP GlnR PHO boxes EMSA DNase footprinting 2415.01 Biología Molecular de Microorganismos 2414.02 Fisiología Bacteriana [EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibrium SI ERA-NET SysMO Project (STREAM) [GEN2006-27745-E/SYS to J.F.M. and FKZ 0315003 to W.W.]; Spanish Comisión Interministerial de Ciencia y Tecnología (CICYT) [BIO2010-16094]. Funding for open access charge: CICYT [BIO2010-16094]. Oxford University Press https://academic.oup.com/nar/article/41/3/1767/2902433 https://hdl.handle.net/10612/17970 |
| title |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor |
| spellingShingle |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor Sola Landa, Alberto Biología Phosphate regulation Nitrogen regulation Streptomyces coelicolor PhoR-PhoP GlnR PHO boxes EMSA DNase footprinting 2415.01 Biología Molecular de Microorganismos 2414.02 Fisiología Bacteriana |
| title_short |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor |
| title_full |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor |
| title_fullStr |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor |
| title_full_unstemmed |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor |
| title_sort |
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor |
| author |
Sola Landa, Alberto |
| author_facet |
Sola Landa, Alberto Rodríguez García, Antonio Amin, Rafat Wohlleben, Wolfgang Martín Martín, Juan Francisco |
| author_role |
author |
| author2 |
Rodríguez García, Antonio Amin, Rafat Wohlleben, Wolfgang Martín Martín, Juan Francisco |
| author2_role |
author author author author |
| topic |
Biología Phosphate regulation Nitrogen regulation Streptomyces coelicolor PhoR-PhoP GlnR PHO boxes EMSA DNase footprinting 2415.01 Biología Molecular de Microorganismos 2414.02 Fisiología Bacteriana |
| topic_facet |
Biología Phosphate regulation Nitrogen regulation Streptomyces coelicolor PhoR-PhoP GlnR PHO boxes EMSA DNase footprinting 2415.01 Biología Molecular de Microorganismos 2414.02 Fisiología Bacteriana |
| description |
[EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibrium |
| publishDate |
2013 |
| format |
article |
| status_str |
publishedVersion |
| url |
https://academic.oup.com/nar/article/41/3/1767/2902433 https://hdl.handle.net/10612/17970 |
| eu_rights_str_mv |
openAccess |
| publisher |
Oxford University Press |
| institution |
Universidad de León |
| collection |
BULERIA. Repositorio Institucional de la Universidad de León |
| reponame_str |
BULERIA. Repositorio Institucional de la Universidad de León |
| instname_str |
Universidad de León |
| _version_ |
1878436967164674049 |
| publishDateSort |
2013 |
| author_browse |
Amin, Rafat Martín Martín, Juan Francisco Rodríguez García, Antonio Sola Landa, Alberto Wohlleben, Wolfgang |
| publisherStr |
Oxford University Press |
| score |
6,924472 |