Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat

Experimental autoimmune encephalomyelitis (EAE), a well-established model of multiple sclerosis, is characterised by microglial activation and lymphocyte infiltration. Induction of EAE in Lewis rats produces an acute monophasic disease characterised by a single peak of disability followed by a spont...

Full description

Bibliographic Details
Authors: Almolda Ardid, Beatriz|||0000-0001-6631-4385, Costa García, Manuela, Montoya Gonzalez, Maria, González, Berta|||0000-0002-1860-3980, Castellano López, Bernardo|||0000-0003-1976-971X
Format: article
Publication Date:2011
Country:España
Institution:Universitat Autònoma de Barcelona
Repository:Dipòsit Digital de Documents de la UAB
Language:English
OAI Identifier:oai:ddd.uab.cat:108866
Online Access:https://ddd.uab.cat/record/108866
https://dx.doi.org/urn:doi:10.1371/journal.pone.0027473
Access Level:Open access
Keyword:Esclerosi múltiple
Multiple sclerosis
Experimental autoimmune encephalomyelitis
id ES_8c0179aedb646cd4fc3ebfa84d6deb8d
oai_identifier_str oai:ddd.uab.cat:108866
network_acronym_str ES
network_name_str España
spelling Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat Almolda Ardid, Beatriz|||0000-0001-6631-4385 Costa García, Manuela Montoya Gonzalez, Maria González, Berta|||0000-0002-1860-3980 Castellano López, Bernardo|||0000-0003-1976-971X Esclerosi múltiple Multiple sclerosis Experimental autoimmune encephalomyelitis Experimental autoimmune encephalomyelitis (EAE), a well-established model of multiple sclerosis, is characterised by microglial activation and lymphocyte infiltration. Induction of EAE in Lewis rats produces an acute monophasic disease characterised by a single peak of disability followed by a spontaneous and complete recovery and a subsequent tolerance to further immunizations. In the current study we have performed a detailed analysis of the dynamics of different lymphocyte populations and cytokine profile along the induction, peak, recovery and post-recovery phases in this paradigm. MBP-injected rats were sacrificed attending exclusively to their clinical score, and the different populations of T-lymphocytes as well as the dynamics of different pro- and anti-inflammatory cytokines were analysed in the spinal cord by flow cytometry, immunohistochemistry and ELISA. Our results revealed that, during the induction and peak phases, in parallel to an increase in symptomatology, the number of CD3+ and CD4+ cells increased progressively, showing a Th1 phenotype, but unexpectedly during recovery, although clinical signs progressively decreased, the number and proportion of CD3+ and CD4+ populations remained unaltered. Interestingly, during this recovery phase, we observed a marked decrease of Th1 and an important increase in Th17 and T-reg cells. Moreover, our results indicate a specific cytokine expression profile along the EAE course characterized by no changes of IL10 and IL17 levels, decrease of IL21 on the peak, and high IL22 levels during the induction and peak phases that markedly decrease during recovery. In summary, these results revealed the existence of a specific pattern of lymphocyte infiltration and cytokine secretion along the different phases of the acute EAE model in Lewis rat that differs from those already described in chronic or relapsing-remitting mouse models, where Th17-cells were found mostly during the peak, suggesting a specific role of these lymphocytes and cytokines in the evolution of this acute EAE model. https://ddd.uab.cat/record/108866 https://dx.doi.org/urn:doi:10.1371/journal.pone.0027473
title Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
spellingShingle Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
Almolda Ardid, Beatriz|||0000-0001-6631-4385
Esclerosi múltiple
Multiple sclerosis
Experimental autoimmune encephalomyelitis
title_short Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
title_full Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
title_fullStr Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
title_full_unstemmed Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
title_sort Increase in Th17 and T-reg lymphocytes and decrease of IL22 correlate with the recovery phase of acute EAE IN rat
author Almolda Ardid, Beatriz|||0000-0001-6631-4385
author_facet Almolda Ardid, Beatriz|||0000-0001-6631-4385
Costa García, Manuela
Montoya Gonzalez, Maria
González, Berta|||0000-0002-1860-3980
Castellano López, Bernardo|||0000-0003-1976-971X
author_role author
author2 Costa García, Manuela
Montoya Gonzalez, Maria
González, Berta|||0000-0002-1860-3980
Castellano López, Bernardo|||0000-0003-1976-971X
author2_role author
author
author
author
topic Esclerosi múltiple
Multiple sclerosis
Experimental autoimmune encephalomyelitis
topic_facet Esclerosi múltiple
Multiple sclerosis
Experimental autoimmune encephalomyelitis
description Experimental autoimmune encephalomyelitis (EAE), a well-established model of multiple sclerosis, is characterised by microglial activation and lymphocyte infiltration. Induction of EAE in Lewis rats produces an acute monophasic disease characterised by a single peak of disability followed by a spontaneous and complete recovery and a subsequent tolerance to further immunizations. In the current study we have performed a detailed analysis of the dynamics of different lymphocyte populations and cytokine profile along the induction, peak, recovery and post-recovery phases in this paradigm. MBP-injected rats were sacrificed attending exclusively to their clinical score, and the different populations of T-lymphocytes as well as the dynamics of different pro- and anti-inflammatory cytokines were analysed in the spinal cord by flow cytometry, immunohistochemistry and ELISA. Our results revealed that, during the induction and peak phases, in parallel to an increase in symptomatology, the number of CD3+ and CD4+ cells increased progressively, showing a Th1 phenotype, but unexpectedly during recovery, although clinical signs progressively decreased, the number and proportion of CD3+ and CD4+ populations remained unaltered. Interestingly, during this recovery phase, we observed a marked decrease of Th1 and an important increase in Th17 and T-reg cells. Moreover, our results indicate a specific cytokine expression profile along the EAE course characterized by no changes of IL10 and IL17 levels, decrease of IL21 on the peak, and high IL22 levels during the induction and peak phases that markedly decrease during recovery. In summary, these results revealed the existence of a specific pattern of lymphocyte infiltration and cytokine secretion along the different phases of the acute EAE model in Lewis rat that differs from those already described in chronic or relapsing-remitting mouse models, where Th17-cells were found mostly during the peak, suggesting a specific role of these lymphocytes and cytokines in the evolution of this acute EAE model.
publishDate 2011
format article
url https://ddd.uab.cat/record/108866
https://dx.doi.org/urn:doi:10.1371/journal.pone.0027473
language eng
eu_rights_str_mv openAccess
institution Universitat Autònoma de Barcelona
collection Dipòsit Digital de Documents de la UAB
reponame_str Dipòsit Digital de Documents de la UAB
instname_str Universitat Autònoma de Barcelona
_version_ 1878736266153951232
publishDateSort 2011
author_browse Almolda Ardid, Beatriz|||0000-0001-6631-4385
Castellano López, Bernardo|||0000-0003-1976-971X
Costa García, Manuela
González, Berta|||0000-0002-1860-3980
Montoya Gonzalez, Maria
score 6,8972664

Similar Items