Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius
28 pages, 4 figures.--Article first published online: 27 FEB 2015
| Autores: | , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versión aceptada para publicación |
| Data de publicação: | 2015 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositório: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/127715 |
| Acesso em linha: | http://hdl.handle.net/10261/127715 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Aspergillus carbonarius Ochratoxin production Oxidative stress Grapes |
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oai:digital.csic.es:10261/127715 |
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Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius Crespo Sempere, Ana Selma Lázaro, Cristina Palumbo, Jeffrey D. González-Candelas, Luis Martínez-Culebras, Pedro V. Aspergillus carbonarius Ochratoxin production Oxidative stress Grapes 28 pages, 4 figures.--Article first published online: 27 FEB 2015 BACKGROUND:There are few studies dealing with the relationship between oxidative stress and ochratoxin A (OTA) biosynthesis. In this work, we analyzed the effect of the oxidant stressor menadione and the antioxidants 3,5-di-tert-butyl-4-hydroxytoluene (BHT), catechin, resveratrol and a polyphenolic extract on growth, generation of reactive oxygen species (ROS), OTA production and gene expression of antioxidant enzymes of Aspergillus carbonarius. RESULTS:Exposure to menadione concentrations higher than 20 µmol L−1 led to increases in ROS and OTA levels and a decrease in growth rate. Exposure to 2.5–10 mmol L−1 BHT also led to higher ROS and OTA levels, although growth rate was only affected above 5 mmol L−1. Naturally occurring concentrations of catechin, resveratrol and polyphenolic extract barely affected growth rate, but they produced widely different effects on OTA production level depending on the antioxidant concentration used. In general, gene expression of antioxidant enzymes superoxide dismutase (SOD) and peroxiredoxin (PRX) was downregulated after exposure to oxidant and antioxidant concentrations that enhanced OTA production level. CONCLUSION:Aspergillus carbonarius responds to oxidative stress, increasing OTA production. Nevertheless, the use of naturally occurring concentrations of antioxidant phenolic compounds to reduce oxidative stress is not a valid approach by itself for OTA contamination control in grapes. A Crespo-Sempere is the recipient of an FPI fellowship from the Spanish Government. This research was supported by grants AGL2005-00707 and AGL2008-04828-C03-02 from the Spanish Government. Peer reviewed John Wiley & Sons http://hdl.handle.net/10261/127715 |
| title |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius |
| spellingShingle |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius Crespo Sempere, Ana Aspergillus carbonarius Ochratoxin production Oxidative stress Grapes |
| title_short |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius |
| title_full |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius |
| title_fullStr |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius |
| title_full_unstemmed |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius |
| title_sort |
Effect of oxidant stressors and phenolic antioxidants on the ochratoxigenic fungus Aspergillus carbonarius |
| author |
Crespo Sempere, Ana |
| author_facet |
Crespo Sempere, Ana Selma Lázaro, Cristina Palumbo, Jeffrey D. González-Candelas, Luis Martínez-Culebras, Pedro V. |
| author_role |
author |
| author2 |
Selma Lázaro, Cristina Palumbo, Jeffrey D. González-Candelas, Luis Martínez-Culebras, Pedro V. |
| author2_role |
author author author author |
| topic |
Aspergillus carbonarius Ochratoxin production Oxidative stress Grapes |
| topic_facet |
Aspergillus carbonarius Ochratoxin production Oxidative stress Grapes |
| description |
28 pages, 4 figures.--Article first published online: 27 FEB 2015 |
| publishDate |
2015 |
| format |
article |
| status_str |
acceptedVersion |
| url |
http://hdl.handle.net/10261/127715 |
| eu_rights_str_mv |
openAccess |
| publisher |
John Wiley & Sons |
| institution |
Consejo Superior de Investigaciones Científicas (CSIC) |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| _version_ |
1878438487205609472 |
| publishDateSort |
2015 |
| author_browse |
Crespo Sempere, Ana González-Candelas, Luis Martínez-Culebras, Pedro V. Palumbo, Jeffrey D. Selma Lázaro, Cristina |
| publisherStr |
John Wiley & Sons |
| score |
6,924472 |