UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein...
| Autores: | , , , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2024 |
| País: | España |
| Recursos: | Universidad Pública de Navarra |
| Repositório: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/52301 |
| Acesso em linha: | https://hdl.handle.net/2454/52301 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Bacillus megaterium Bacillus thuringiensis Bioinsecticide formulations Biological control Insect pests Microencapsulation Priestia megaterium Vip3 proteins |
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oai:academica-e.unavarra.es:2454/52301 |
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UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium Palma Dovis, Leopoldo Ruiz de Escudero Fuentemilla, Íñigo Mañeru Oria, Francisco Javier Berry, Colin Caballero Murillo, Primitivo Bacillus megaterium Bacillus thuringiensis Bioinsecticide formulations Biological control Insect pests Microencapsulation Priestia megaterium Vip3 proteins In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light. This research was funded by the Spanish Ministry of Science and Innovation grant number AGL2009-13340-C02 and by the Universidad Pública de Navarra. Leopoldo Palma would like to express gratitude to the Spanish Government-Universities Ministry, the NextGenerationEU and Recovery, Transformation, and Resilience plans for funding his awarded María Zambrano contract (ref. ZA21-003). Elsevier https://hdl.handle.net/2454/52301 |
| title |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium |
| spellingShingle |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium Palma Dovis, Leopoldo Bacillus megaterium Bacillus thuringiensis Bioinsecticide formulations Biological control Insect pests Microencapsulation Priestia megaterium Vip3 proteins |
| title_short |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium |
| title_full |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium |
| title_fullStr |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium |
| title_full_unstemmed |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium |
| title_sort |
UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium |
| author |
Palma Dovis, Leopoldo |
| author_facet |
Palma Dovis, Leopoldo Ruiz de Escudero Fuentemilla, Íñigo Mañeru Oria, Francisco Javier Berry, Colin Caballero Murillo, Primitivo |
| author_role |
author |
| author2 |
Ruiz de Escudero Fuentemilla, Íñigo Mañeru Oria, Francisco Javier Berry, Colin Caballero Murillo, Primitivo |
| author2_role |
author author author author |
| topic |
Bacillus megaterium Bacillus thuringiensis Bioinsecticide formulations Biological control Insect pests Microencapsulation Priestia megaterium Vip3 proteins |
| topic_facet |
Bacillus megaterium Bacillus thuringiensis Bioinsecticide formulations Biological control Insect pests Microencapsulation Priestia megaterium Vip3 proteins |
| description |
In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light. |
| publishDate |
2024 |
| format |
article |
| status_str |
publishedVersion |
| url |
https://hdl.handle.net/2454/52301 |
| eu_rights_str_mv |
openAccess |
| publisher |
Elsevier |
| institution |
Universidad Pública de Navarra |
| collection |
Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| reponame_str |
Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| instname_str |
Universidad Pública de Navarra |
| _version_ |
1878439111990181889 |
| publishDateSort |
2024 |
| author_browse |
Berry, Colin Caballero Murillo, Primitivo Mañeru Oria, Francisco Javier Palma Dovis, Leopoldo Ruiz de Escudero Fuentemilla, Íñigo |
| publisherStr |
Elsevier |
| score |
6,924472 |