Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)

Lima Cabello, Elena; Escudero-Feliú, Julia; Peralta, Andreína; García-Fernandez, Pedro; Siddique, Kadambot H. M.; Singh, Karam B.; Núñez, María Isabel; León, Josefa; Jiménez-López, José Carlos

Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)

Narrow-leafed lupin (NLL; Lupinus angustifolius L.) has multiple nutraceutical properties that may result from unique structural features of β-conglutin proteins, such as the mobile arm at the N-terminal, a structural domain rich in α-helices. A similar domain has not been found in other vicilin pro...

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Authors:	Lima Cabello, Elena, Escudero-Feliú, Julia, Peralta, Andreína, García-Fernandez, Pedro, Siddique, Kadambot H. M., Singh, Karam B., Núñez, María Isabel, León, Josefa, Jiménez-López, José Carlos
Format:	article
Status:	Published version
Publication Date:	2023
Country:	España
Institution:	Consejo Superior de Investigaciones Científicas (CSIC)
Repository:	DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:	oai:digital.csic.es:10261/308130
Online Access:	http://hdl.handle.net/10261/308130
Access Level:	Open access
Keyword:	Legumes Sweet lupin Vicilin Anti-inflammatory Molecular nutraceutics Redox regulatory capacity Mobile arm structural domain Truncated β-conglutins

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network_acronym_str	ES
network_name_str	España
spelling	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.) Lima Cabello, Elena Escudero-Feliú, Julia Peralta, Andreína García-Fernandez, Pedro Siddique, Kadambot H. M. Singh, Karam B. Núñez, María Isabel León, Josefa Jiménez-López, José Carlos Legumes Sweet lupin Vicilin Anti-inflammatory Molecular nutraceutics Redox regulatory capacity Mobile arm structural domain Truncated β-conglutins Narrow-leafed lupin (NLL; Lupinus angustifolius L.) has multiple nutraceutical properties that may result from unique structural features of β-conglutin proteins, such as the mobile arm at the N-terminal, a structural domain rich in α-helices. A similar domain has not been found in other vicilin proteins of legume species. We used affinity chromatography to purify recombinant complete and truncated (without the mobile arm domain, tβ5 and tβ7) forms of NLL β5 and β7 conglutin proteins. We then used biochemical and molecular biology techniques in ex vivo and in vitro systems to evaluate their anti-inflammatory activity and antioxidant capacity. The complete β5 and β7 conglutin proteins decreased pro-inflammatory mediator levels (e.g., nitric oxide), mRNA expression levels (iNOS, TNFα, IL-1β), and the protein levels of pro-inflammatory cytokine TNF-α, interleukins (IL-1β, IL-2, IL-6, IL-8, IL-12, IL-17, IL-27), and other mediators (INFγ, MOP, S-TNF-R1/-R2, and TWEAK), and exerted a regulatory oxidative balance effect in cells as demonstrated in glutathione, catalase, and superoxide dismutase assays. The truncated tβ5 and tβ7 conglutin proteins did not have these molecular effects. These results suggest that β5 and β7 conglutins have potential as functional food components due to their anti-inflammatory and oxidative cell state regulatory properties, and that the mobile arm of NLL β-conglutin proteins is a key domain in the development of nutraceutical properties, making NLL β5 and β7 excellent innovative candidates as functional foods. This study was funded by the European Research Program MARIE CURIE (FP7-PEOPLE-2011-IOF), grant ref. PIOF-GA-2011-301550; the Spanish Ministry of Economy, Industry and Competitiveness (Ramon y Cajal Research Program), grant ref. RYC-2014-16536; the CSIC intramural research program, grant ref. 202240I002; the Spanish Ministry of Science and Innovation, grant ref. number CPP2021-008989. Peer reviewed Multidisciplinary Digital Publishing Institute http://hdl.handle.net/10261/308130
title	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)
spellingShingle	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.) Lima Cabello, Elena Legumes Sweet lupin Vicilin Anti-inflammatory Molecular nutraceutics Redox regulatory capacity Mobile arm structural domain Truncated β-conglutins
title_short	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)
title_full	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)
title_fullStr	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)
title_full_unstemmed	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)
title_sort	Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)
author	Lima Cabello, Elena
author_facet	Lima Cabello, Elena Escudero-Feliú, Julia Peralta, Andreína García-Fernandez, Pedro Siddique, Kadambot H. M. Singh, Karam B. Núñez, María Isabel León, Josefa Jiménez-López, José Carlos
author_role	author
author2	Escudero-Feliú, Julia Peralta, Andreína García-Fernandez, Pedro Siddique, Kadambot H. M. Singh, Karam B. Núñez, María Isabel León, Josefa Jiménez-López, José Carlos
author2_role	author author author author author author author author
topic	Legumes Sweet lupin Vicilin Anti-inflammatory Molecular nutraceutics Redox regulatory capacity Mobile arm structural domain Truncated β-conglutins
topic_facet	Legumes Sweet lupin Vicilin Anti-inflammatory Molecular nutraceutics Redox regulatory capacity Mobile arm structural domain Truncated β-conglutins
description	Narrow-leafed lupin (NLL; Lupinus angustifolius L.) has multiple nutraceutical properties that may result from unique structural features of β-conglutin proteins, such as the mobile arm at the N-terminal, a structural domain rich in α-helices. A similar domain has not been found in other vicilin proteins of legume species. We used affinity chromatography to purify recombinant complete and truncated (without the mobile arm domain, tβ5 and tβ7) forms of NLL β5 and β7 conglutin proteins. We then used biochemical and molecular biology techniques in ex vivo and in vitro systems to evaluate their anti-inflammatory activity and antioxidant capacity. The complete β5 and β7 conglutin proteins decreased pro-inflammatory mediator levels (e.g., nitric oxide), mRNA expression levels (iNOS, TNFα, IL-1β), and the protein levels of pro-inflammatory cytokine TNF-α, interleukins (IL-1β, IL-2, IL-6, IL-8, IL-12, IL-17, IL-27), and other mediators (INFγ, MOP, S-TNF-R1/-R2, and TWEAK), and exerted a regulatory oxidative balance effect in cells as demonstrated in glutathione, catalase, and superoxide dismutase assays. The truncated tβ5 and tβ7 conglutin proteins did not have these molecular effects. These results suggest that β5 and β7 conglutins have potential as functional food components due to their anti-inflammatory and oxidative cell state regulatory properties, and that the mobile arm of NLL β-conglutin proteins is a key domain in the development of nutraceutical properties, making NLL β5 and β7 excellent innovative candidates as functional foods.
publishDate	2023
format	article
status_str	publishedVersion
url	http://hdl.handle.net/10261/308130
eu_rights_str_mv	openAccess
publisher	Multidisciplinary Digital Publishing Institute
institution	Consejo Superior de Investigaciones Científicas (CSIC)
collection	DIGITAL.CSIC. Repositorio Institucional del CSIC
reponame_str	DIGITAL.CSIC. Repositorio Institucional del CSIC
instname_str	Consejo Superior de Investigaciones Científicas (CSIC)
_version_	1878439488282165248
publishDateSort	2023
author_browse	Escudero-Feliú, Julia García-Fernandez, Pedro Jiménez-López, José Carlos León, Josefa Lima Cabello, Elena Núñez, María Isabel Peralta, Andreína Siddique, Kadambot H. M. Singh, Karam B.
publisherStr	Multidisciplinary Digital Publishing Institute
score	6.9303427

Beta conglutins’ unique mobile arm Is a key structural domain involved in molecular nutraceutical properties of narrow-leafed Lupin (Lupinus angustifolius L.)

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