Genome Editing by CRISPR/Cas9 in Polyploids
CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing require...
| Autores: | , , |
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| Tipo de recurso: | otro |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/372213 |
| Acceso en línea: | http://hdl.handle.net/10261/372213 https://api.elsevier.com/content/abstract/scopus_id/85147186818 |
| Access Level: | acceso abierto |
| Palabra clave: | CRISPR/Cas9 Genome editing Homoeologous genes Off-targets Polyploids sgRNA |
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oai:digital.csic.es:10261/372213 |
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Genome Editing by CRISPR/Cas9 in Polyploids Sánchez-Gómez, Carlos Posé, David Martín-Pizarro, Carmen CRISPR/Cas9 Genome editing Homoeologous genes Off-targets Polyploids sgRNA CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing requires more rigorous work and specific needs to assess successful genome editing. Here, we describe a general stepwise protocol to select target sites, design sgRNAs, indicate vector requirements, and screen CRISPR/Cas9-mediated genome editing in polyploid species. CSG was supported by a grant from the Spanish Ministries of Science and Innovation (MICINN, RTI2018-09309-A-I00). CMP was supported by a grant from the European Research Council (ERC-2014-StG 638134). Peer reviewed Springer Nature http://hdl.handle.net/10261/372213 https://api.elsevier.com/content/abstract/scopus_id/85147186818 |
| title |
Genome Editing by CRISPR/Cas9 in Polyploids |
| spellingShingle |
Genome Editing by CRISPR/Cas9 in Polyploids Sánchez-Gómez, Carlos CRISPR/Cas9 Genome editing Homoeologous genes Off-targets Polyploids sgRNA |
| title_short |
Genome Editing by CRISPR/Cas9 in Polyploids |
| title_full |
Genome Editing by CRISPR/Cas9 in Polyploids |
| title_fullStr |
Genome Editing by CRISPR/Cas9 in Polyploids |
| title_full_unstemmed |
Genome Editing by CRISPR/Cas9 in Polyploids |
| title_sort |
Genome Editing by CRISPR/Cas9 in Polyploids |
| author |
Sánchez-Gómez, Carlos |
| author_facet |
Sánchez-Gómez, Carlos Posé, David Martín-Pizarro, Carmen |
| author_role |
author |
| author2 |
Posé, David Martín-Pizarro, Carmen |
| author2_role |
author author |
| topic |
CRISPR/Cas9 Genome editing Homoeologous genes Off-targets Polyploids sgRNA |
| topic_facet |
CRISPR/Cas9 Genome editing Homoeologous genes Off-targets Polyploids sgRNA |
| description |
CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing requires more rigorous work and specific needs to assess successful genome editing. Here, we describe a general stepwise protocol to select target sites, design sgRNAs, indicate vector requirements, and screen CRISPR/Cas9-mediated genome editing in polyploid species. |
| publishDate |
2023 |
| format |
other |
| status_str |
acceptedVersion |
| url |
http://hdl.handle.net/10261/372213 https://api.elsevier.com/content/abstract/scopus_id/85147186818 |
| eu_rights_str_mv |
openAccess |
| publisher |
Springer Nature |
| institution |
Consejo Superior de Investigaciones Científicas (CSIC) |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
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1878439589435146241 |
| publishDateSort |
2023 |
| author_browse |
Martín-Pizarro, Carmen Posé, David Sánchez-Gómez, Carlos |
| publisherStr |
Springer Nature |
| score |
6,924472 |