Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain

[EN]Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulati...

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Autores: Flores Félix, José David, Carro García, Lorena, Cerda Castillo, María Eugenia, Squartini, Andrea, Rivas González, Raúl, Velázquez Pérez, María Encarnación
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/145538
Acceso en línea:http://hdl.handle.net/10366/145538
Access Level:acceso abierto
Palabra clave:Pea
Pisum sativum
Rhizobium laguerreae
Symbiosis
Quorum sensing
Nodulation effectiveness
Nitrogen fixation
2414 Microbiología
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spelling Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain Flores Félix, José David Carro García, Lorena Cerda Castillo, María Eugenia Squartini, Andrea Rivas González, Raúl Velázquez Pérez, María Encarnación Pea Pisum sativum Rhizobium laguerreae Symbiosis Quorum sensing Nodulation effectiveness Nitrogen fixation 2414 Microbiología [EN]Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. laguerreae sv. viciae. Representative strains of these clusters were able to produce cellulose and cellulases, which are two key molecules in the legume infection process. They formed biofilms and produced acyl-homoserine lactones (AHLs), which are involved in the quorum sensing regulation process. They also exhibited several plant growth promotion mechanisms, including phosphate solubilization, siderophore, and indole acetic acid production and symbiotic atmospheric nitrogen fixation. All strains showed high symbiotic efficiency on pea plants, indicating that strains of R. laguerreae sv. viciae are promising candidates for the biofertilization of this legume worldwide. MDPI http://hdl.handle.net/10366/145538
title Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
spellingShingle Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
Flores Félix, José David
Pea
Pisum sativum
Rhizobium laguerreae
Symbiosis
Quorum sensing
Nodulation effectiveness
Nitrogen fixation
2414 Microbiología
title_short Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
title_full Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
title_fullStr Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
title_full_unstemmed Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
title_sort Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
author Flores Félix, José David
author_facet Flores Félix, José David
Carro García, Lorena
Cerda Castillo, María Eugenia
Squartini, Andrea
Rivas González, Raúl
Velázquez Pérez, María Encarnación
author_role author
author2 Carro García, Lorena
Cerda Castillo, María Eugenia
Squartini, Andrea
Rivas González, Raúl
Velázquez Pérez, María Encarnación
author2_role author
author
author
author
author
topic Pea
Pisum sativum
Rhizobium laguerreae
Symbiosis
Quorum sensing
Nodulation effectiveness
Nitrogen fixation
2414 Microbiología
topic_facet Pea
Pisum sativum
Rhizobium laguerreae
Symbiosis
Quorum sensing
Nodulation effectiveness
Nitrogen fixation
2414 Microbiología
description [EN]Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. laguerreae sv. viciae. Representative strains of these clusters were able to produce cellulose and cellulases, which are two key molecules in the legume infection process. They formed biofilms and produced acyl-homoserine lactones (AHLs), which are involved in the quorum sensing regulation process. They also exhibited several plant growth promotion mechanisms, including phosphate solubilization, siderophore, and indole acetic acid production and symbiotic atmospheric nitrogen fixation. All strains showed high symbiotic efficiency on pea plants, indicating that strains of R. laguerreae sv. viciae are promising candidates for the biofertilization of this legume worldwide.
publishDate 2020
format article
status_str publishedVersion
url http://hdl.handle.net/10366/145538
eu_rights_str_mv openAccess
publisher MDPI
institution Universidad de Salamanca (USAL)
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname_str Universidad de Salamanca (USAL)
_version_ 1878440099663839233
publishDateSort 2020
author_browse Carro García, Lorena
Cerda Castillo, María Eugenia
Flores Félix, José David
Rivas González, Raúl
Squartini, Andrea
Velázquez Pérez, María Encarnación
publisherStr MDPI
score 6,924472

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