Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces
37 p.-8 fig.-2 tab.
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/175266 |
| Acceso en línea: | http://hdl.handle.net/10261/175266 |
| Access Level: | acceso abierto |
| Palabra clave: | Phasins Polyhydroxyalkanoates Biosurfactants Pseudomonas putida |
| id |
ES_cfe56431e15dfabb9c8fbaf2dff0456a |
|---|---|
| oai_identifier_str |
oai:digital.csic.es:10261/175266 |
| network_acronym_str |
ES |
| network_name_str |
España |
| spelling |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces Mato, Aranzazu Tarazona, Natalia A. Hidalgo, Alberto Cruz, Antonio Jiménez, Mercedes Pérez-Gil, Jesús Prieto, María Auxiliadora Phasins Polyhydroxyalkanoates Biosurfactants Pseudomonas putida 37 p.-8 fig.-2 tab. Phasins, the major proteins coating polyhydroxyalkanoate (PHA) granules, have been proposed as suitable biosurfactants for multiple applications because of their amphiphilic nature. In this work, we analyzed the interfacial activity of the amphiphilic α-helical phasin PhaF from Pseudomonas putida KT2440 at different hydrophobic–hydrophilic interfacial environments. The binding of PhaF to surfaces containing PHA or phospholipids, postulated as structural components of PHA granules, was confirmed in vitro using supported lipid bilayers and confocal microscopy, with polyhydroxyoctanoate-co-hexanoate P(HO-co-HHx) and Escherichia coli lipid extract as model systems. The surfactant-like capabilities of PhaF were determined by measuring changes in surface pressure in Langmuir devices. PhaF spontaneously adsorbed at the air–water interface, reducing the surface tension from 72 mN/m (water surface tension at 25 °C) to 50 mN/m. The differences in the adsorption of the protein in the presence of different phospholipid films showed a marked preference for phosphatidylglycerol species, such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. The PHA-binding domain of PhaF (BioF) conserved a similar surface activity to PhaF, suggesting that it is responsible for the surfactant properties of the whole protein. These new findings not only increase our knowledge about the role of phasins in the PHA machinery but also open new outlooks for the application of these proteins as biosurfactants. This work was supported by the European Union’s Horizon 2020 Research and innovation programme, grant agreement no. 633962 (P4SB), the Community of Madrid (P2013/MIT2807) and the Spanish Ministry of Science, Innovation and Universities (BIO2017-83448-R, BIO2015-67930-R). Peer reviewed American Chemical Society http://hdl.handle.net/10261/175266 |
| title |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces |
| spellingShingle |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces Mato, Aranzazu Phasins Polyhydroxyalkanoates Biosurfactants Pseudomonas putida |
| title_short |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces |
| title_full |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces |
| title_fullStr |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces |
| title_full_unstemmed |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces |
| title_sort |
Interfacial activity of phasin PhaF from Pseudomonas putida KT2440 at hydrophobic-hydrophilic biointerfaces |
| author |
Mato, Aranzazu |
| author_facet |
Mato, Aranzazu Tarazona, Natalia A. Hidalgo, Alberto Cruz, Antonio Jiménez, Mercedes Pérez-Gil, Jesús Prieto, María Auxiliadora |
| author_role |
author |
| author2 |
Tarazona, Natalia A. Hidalgo, Alberto Cruz, Antonio Jiménez, Mercedes Pérez-Gil, Jesús Prieto, María Auxiliadora |
| author2_role |
author author author author author author |
| topic |
Phasins Polyhydroxyalkanoates Biosurfactants Pseudomonas putida |
| topic_facet |
Phasins Polyhydroxyalkanoates Biosurfactants Pseudomonas putida |
| description |
37 p.-8 fig.-2 tab. |
| publishDate |
2019 |
| format |
article |
| status_str |
acceptedVersion |
| url |
http://hdl.handle.net/10261/175266 |
| eu_rights_str_mv |
openAccess |
| publisher |
American Chemical Society |
| institution |
Consejo Superior de Investigaciones Científicas (CSIC) |
| collection |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
| instname_str |
Consejo Superior de Investigaciones Científicas (CSIC) |
| _version_ |
1878441030005555200 |
| publishDateSort |
2019 |
| author_browse |
Cruz, Antonio Hidalgo, Alberto Jiménez, Mercedes Mato, Aranzazu Prieto, María Auxiliadora Pérez-Gil, Jesús Tarazona, Natalia A. |
| publisherStr |
American Chemical Society |
| score |
6,9303427 |