Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals
Background The effect of maraviroc on the maintenance and the function of HIV-1-specific T cell responses remains unknown. Methods Subjects recently infected with HIV-1 were randomized to receive anti-retroviral treatment with or without maraviroc intensification for 48 weeks, and were monitored up...
| Autores: | , , , , , , , , , , , , , , , |
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| Tipo de documento: | artigo |
| Data de publicação: | 2014 |
| País: | España |
| Recursos: | UVic-UCC |
| Repositório: | RiUVic. Repositori institucional de la UVic-UCC |
| OAI Identifier: | oai:dspace.uvic.cat:10854/2737 |
| Acesso em linha: | http://hdl.handle.net/10854/2737 https://doi.org/10.1371/journal.pone.0087334 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Sida -- Tractament |
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oai:dspace.uvic.cat:10854/2737 |
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Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals Kawana-Tachikawa, A. Llibre, Josep M. Bravo, Isabel Escrig, R. Mothe, Beatriz Puig, Jordi Puertas, M.C. Martinez Picado, Francisco Javier Blanco, Julià Manzardo, C. Miró, J.M. Iwamoto, A. Pozniak, A.L. Gatell, J.M. Clotet, Bonaventura Brander, Christian Sida -- Tractament Background The effect of maraviroc on the maintenance and the function of HIV-1-specific T cell responses remains unknown. Methods Subjects recently infected with HIV-1 were randomized to receive anti-retroviral treatment with or without maraviroc intensification for 48 weeks, and were monitored up to week 60. PBMC and in vitro-expanded T cells were tested for responses to the entire HIV proteome by ELISpot analyses. Intracellular cytokine staining assays were conducted to monitor the (poly)-functionality of HIV-1-specific T cells. Analyses were performed at baseline and week 24 after treatment start, and at week 60 (3 months after maraviroc discontinuation). Results Maraviroc intensification was associated with a slower decay of virus-specific T cell responses over time compared to the non-intensified regimen in both direct ex-vivo as well as in in-vitro expanded cells. The effector function profiles of virus-specific CD8+ T cells were indistinguishable between the two arms and did not change over time between the groups. Conclusions Maraviroc did not negatively impact any of the measured parameters, but was rather associated with a prolonged maintenance of HIV-1-specific T cell responses. Maraviroc, in addition to its original effect as viral entry inhibitor, may provide an additional benefit on the maintenance of virus-specific T cells which may be especially important for future viral eradication strategies. Public Library of Science http://hdl.handle.net/10854/2737 https://doi.org/10.1371/journal.pone.0087334 |
| title |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals |
| spellingShingle |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals Kawana-Tachikawa, A. Sida -- Tractament |
| title_short |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals |
| title_full |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals |
| title_fullStr |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals |
| title_full_unstemmed |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals |
| title_sort |
Effect of Maraviroc Intensification on HIV-1-Specific T Cell Immunity in Recently HIV-1-Infected Individuals |
| author |
Kawana-Tachikawa, A. |
| author_facet |
Kawana-Tachikawa, A. Llibre, Josep M. Bravo, Isabel Escrig, R. Mothe, Beatriz Puig, Jordi Puertas, M.C. Martinez Picado, Francisco Javier Blanco, Julià Manzardo, C. Miró, J.M. Iwamoto, A. Pozniak, A.L. Gatell, J.M. Clotet, Bonaventura Brander, Christian |
| author_role |
author |
| author2 |
Llibre, Josep M. Bravo, Isabel Escrig, R. Mothe, Beatriz Puig, Jordi Puertas, M.C. Martinez Picado, Francisco Javier Blanco, Julià Manzardo, C. Miró, J.M. Iwamoto, A. Pozniak, A.L. Gatell, J.M. Clotet, Bonaventura Brander, Christian |
| author2_role |
author author author author author author author author author author author author author author author |
| topic |
Sida -- Tractament |
| topic_facet |
Sida -- Tractament |
| description |
Background The effect of maraviroc on the maintenance and the function of HIV-1-specific T cell responses remains unknown. Methods Subjects recently infected with HIV-1 were randomized to receive anti-retroviral treatment with or without maraviroc intensification for 48 weeks, and were monitored up to week 60. PBMC and in vitro-expanded T cells were tested for responses to the entire HIV proteome by ELISpot analyses. Intracellular cytokine staining assays were conducted to monitor the (poly)-functionality of HIV-1-specific T cells. Analyses were performed at baseline and week 24 after treatment start, and at week 60 (3 months after maraviroc discontinuation). Results Maraviroc intensification was associated with a slower decay of virus-specific T cell responses over time compared to the non-intensified regimen in both direct ex-vivo as well as in in-vitro expanded cells. The effector function profiles of virus-specific CD8+ T cells were indistinguishable between the two arms and did not change over time between the groups. Conclusions Maraviroc did not negatively impact any of the measured parameters, but was rather associated with a prolonged maintenance of HIV-1-specific T cell responses. Maraviroc, in addition to its original effect as viral entry inhibitor, may provide an additional benefit on the maintenance of virus-specific T cells which may be especially important for future viral eradication strategies. |
| publishDate |
2014 |
| format |
article |
| url |
http://hdl.handle.net/10854/2737 https://doi.org/10.1371/journal.pone.0087334 |
| eu_rights_str_mv |
openAccess |
| publisher |
Public Library of Science |
| institution |
UVic-UCC |
| collection |
RiUVic. Repositori institucional de la UVic-UCC |
| reponame_str |
RiUVic. Repositori institucional de la UVic-UCC |
| instname_str |
UVic-UCC |
| _version_ |
1878441134920826880 |
| publishDateSort |
2014 |
| author_browse |
Blanco, Julià Brander, Christian Bravo, Isabel Clotet, Bonaventura Escrig, R. Gatell, J.M. Iwamoto, A. Kawana-Tachikawa, A. Llibre, Josep M. Manzardo, C. Martinez Picado, Francisco Javier Miró, J.M. Mothe, Beatriz Pozniak, A.L. Puertas, M.C. Puig, Jordi |
| publisherStr |
Public Library of Science |
| score |
6,924472 |