Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey.
Giardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causin...
| Autores: | , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Data de publicação: | 2020 |
| País: | España |
| Recursos: | Instituto de Salud Carlos III (ISCIII) |
| Repositório: | Repisalud |
| Idioma: | inglês |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/10486 |
| Acesso em linha: | http://hdl.handle.net/20.500.12105/10486 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Multilocus Sequence Typing Adolescent Adult Aged Child Child, Preschool Cytoskeletal Proteins Female Genotype Giardia lamblia Giardiasis Glutamate Dehydrogenase Humans Iran Male Middle Aged Phylogeny Protozoan Proteins Triose-Phosphate Isomerase Young Adult |
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oai:repisalud.isciii.es:20.500.12105/10486 |
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Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. Rafiei, Abdollah Baghlaninezhad, Raheleh Panabad, Esmat Beiromvand, Molouk Köster, Pamela Carolina Bailo-Barroso, Begoña Hernandez-De-Mingo, Marta Carmena, David Multilocus Sequence Typing Adolescent Adult Aged Child Child, Preschool Cytoskeletal Proteins Female Genotype Giardia lamblia Giardiasis Glutamate Dehydrogenase Humans Iran Male Middle Aged Phylogeny Protozoan Proteins Triose-Phosphate Isomerase Young Adult Giardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causing human infections in Shushtar County, Southwestern Iran. Individual faecal specimens were collected from 1,163 individuals (male/female ratio: 0.9; age range 2-75 years) with (n = 258) and without (n = 905) gastrointestinal symptoms living in rural and urban settings during the period 2017-2018. Conventional (sucrose flotation and microscopy) methods were used for the initial detection of G. duodenalis cysts in faecal specimens. Microscopy-positive samples were confirmed by PCR amplification and sequencing of the small subunit rRNA (ssu rRNA) gene of the parasite. A multilocus genotyping (MLG) scheme targeting the triose phosphate isomerase (tpi), the glutamate dehydrogenase (gdh), and the beta-giardin (bg) genes was used for genotyping purposes. Giardia duodenalis cysts were detected in 7.7% (90/1,163) of samples by microscopy, of which 82 were confirmed by ssu-PCR. Successful amplification and sequencing results were obtained for 23.2% (19/82), 9.8% (8/82), and 8.5% (7/82) of the confirmed samples at the tpi, gdh, and bg loci, respectively. MLG data for the three loci were available for two samples only. Out of the 24 samples genotyped at any loci, 50% (12/24) were identified as assemblage A and the remaining half as assemblage B. Overall, AII was the most prevalent sub-assemblage detected (41.7%, 10/24), followed by BIII (25.0%, 6/24), discordant BIII/BIV (5/24) or AII/AIII (2/24) sequences, and BIV (1/24). No significant correlation was demonstrated between a given assemblage/sub-assemblage and the occurrence of clinical symptoms. No genotypes adapted to animal hosts other than humans (e.g. assemblages C-F) were found circulating in the investigated human population, suggesting that transmission of human giardiasis in this Iranian region is primarily of anthroponotic nature. Further molecular-based studies are needed to confirm and expand these results, and to ascertain the presence and public health relevance of the parasite in environmental (e.g. drinking water) samples. Public Library of Science (PLOS) http://hdl.handle.net/20.500.12105/10486 |
| title |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. |
| spellingShingle |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. Rafiei, Abdollah Multilocus Sequence Typing Adolescent Adult Aged Child Child, Preschool Cytoskeletal Proteins Female Genotype Giardia lamblia Giardiasis Glutamate Dehydrogenase Humans Iran Male Middle Aged Phylogeny Protozoan Proteins Triose-Phosphate Isomerase Young Adult |
| title_short |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. |
| title_full |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. |
| title_fullStr |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. |
| title_full_unstemmed |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. |
| title_sort |
Multilocus genotyping of Giardia duodenalis in Southwestern Iran. A community survey. |
| author |
Rafiei, Abdollah |
| author_facet |
Rafiei, Abdollah Baghlaninezhad, Raheleh Panabad, Esmat Beiromvand, Molouk Köster, Pamela Carolina Bailo-Barroso, Begoña Hernandez-De-Mingo, Marta Carmena, David |
| author_role |
author |
| author2 |
Baghlaninezhad, Raheleh Panabad, Esmat Beiromvand, Molouk Köster, Pamela Carolina Bailo-Barroso, Begoña Hernandez-De-Mingo, Marta Carmena, David |
| author2_role |
author author author author author author author |
| topic |
Multilocus Sequence Typing Adolescent Adult Aged Child Child, Preschool Cytoskeletal Proteins Female Genotype Giardia lamblia Giardiasis Glutamate Dehydrogenase Humans Iran Male Middle Aged Phylogeny Protozoan Proteins Triose-Phosphate Isomerase Young Adult |
| topic_facet |
Multilocus Sequence Typing Adolescent Adult Aged Child Child, Preschool Cytoskeletal Proteins Female Genotype Giardia lamblia Giardiasis Glutamate Dehydrogenase Humans Iran Male Middle Aged Phylogeny Protozoan Proteins Triose-Phosphate Isomerase Young Adult |
| description |
Giardia duodenalis is one of the main enteric pathogens associated with diarrheal disease. In developing countries, giardiasis is a major public health concern, particularly in children under five years of age. This study aimed to evaluate the occurrence and genetic diversity of G. duodenalis causing human infections in Shushtar County, Southwestern Iran. Individual faecal specimens were collected from 1,163 individuals (male/female ratio: 0.9; age range 2-75 years) with (n = 258) and without (n = 905) gastrointestinal symptoms living in rural and urban settings during the period 2017-2018. Conventional (sucrose flotation and microscopy) methods were used for the initial detection of G. duodenalis cysts in faecal specimens. Microscopy-positive samples were confirmed by PCR amplification and sequencing of the small subunit rRNA (ssu rRNA) gene of the parasite. A multilocus genotyping (MLG) scheme targeting the triose phosphate isomerase (tpi), the glutamate dehydrogenase (gdh), and the beta-giardin (bg) genes was used for genotyping purposes. Giardia duodenalis cysts were detected in 7.7% (90/1,163) of samples by microscopy, of which 82 were confirmed by ssu-PCR. Successful amplification and sequencing results were obtained for 23.2% (19/82), 9.8% (8/82), and 8.5% (7/82) of the confirmed samples at the tpi, gdh, and bg loci, respectively. MLG data for the three loci were available for two samples only. Out of the 24 samples genotyped at any loci, 50% (12/24) were identified as assemblage A and the remaining half as assemblage B. Overall, AII was the most prevalent sub-assemblage detected (41.7%, 10/24), followed by BIII (25.0%, 6/24), discordant BIII/BIV (5/24) or AII/AIII (2/24) sequences, and BIV (1/24). No significant correlation was demonstrated between a given assemblage/sub-assemblage and the occurrence of clinical symptoms. No genotypes adapted to animal hosts other than humans (e.g. assemblages C-F) were found circulating in the investigated human population, suggesting that transmission of human giardiasis in this Iranian region is primarily of anthroponotic nature. Further molecular-based studies are needed to confirm and expand these results, and to ascertain the presence and public health relevance of the parasite in environmental (e.g. drinking water) samples. |
| publishDate |
2020 |
| format |
article |
| url |
http://hdl.handle.net/20.500.12105/10486 |
| language |
eng |
| eu_rights_str_mv |
openAccess |
| publisher |
Public Library of Science (PLOS) |
| institution |
Instituto de Salud Carlos III (ISCIII) |
| collection |
Repisalud |
| reponame_str |
Repisalud |
| instname_str |
Instituto de Salud Carlos III (ISCIII) |
| _version_ |
1878442037375664128 |
| publishDateSort |
2020 |
| author_browse |
Baghlaninezhad, Raheleh Bailo-Barroso, Begoña Beiromvand, Molouk Carmena, David Hernandez-De-Mingo, Marta Köster, Pamela Carolina Panabad, Esmat Rafiei, Abdollah |
| publisherStr |
Public Library of Science (PLOS) |
| score |
6,924472 |