Markers of endothelial damage in patients with chronic kidney disease on hemodialysis
Patients with Stage 5 chronic kidney disease who are on hemodialysis (HD) remain in a chronic inflammatory state, characterized by the accumulation of uremic toxins that induce endothelial damage and cardiovascular disease (CVD). Our aim was to examine microvesicles (MVs), monocyte subpopulations, a...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat de Lleida (UdL) |
| Repositorio: | Repositori Obert UdL |
| OAI Identifier: | oai:repositori.udl.cat:10459.1/64518 |
| Acceso en línea: | https://doi.org/10.1152/ajprenal.00013.2016 http://hdl.handle.net/10459.1/64518 |
| Access Level: | acceso abierto |
| Palabra clave: | Chronic kidney disease Cardiovascular disease Diabetes mellitus |
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oai:repositori.udl.cat:10459.1/64518 |
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Markers of endothelial damage in patients with chronic kidney disease on hemodialysis Carmona, Andrés Agüera, Maria L. Luna-Ruiz, Carlos Buendía, Paula Calleros-Basilio, Laura García-Jerez, Andrea Rodríguez-Puyol, Manuel Arias, Manuel Arias-Guillen, Marta Arriba, Gabriel de Ballarín, José Bernis, Carmen Fernández i Giráldez, Elvira García-Rebollo, Sagrario Mancha, Javier Peso, Gloria del Pérez, Estefanía Poch, Esteban Portolés, José M. Rodríguez-Puyol, Diego Sánchez-Villanueva, Rafael Sarró, Felipe Torres, Armando Martín-Malo, Alejandro Aljama, Pedro Ramírez, Rafael Carracedo, Júlia Chronic kidney disease Cardiovascular disease Diabetes mellitus Patients with Stage 5 chronic kidney disease who are on hemodialysis (HD) remain in a chronic inflammatory state, characterized by the accumulation of uremic toxins that induce endothelial damage and cardiovascular disease (CVD). Our aim was to examine microvesicles (MVs), monocyte subpopulations, and angiopoietins (Ang) to identify prognostic markers in HD patients with or without diabetes mellitus (DM). A total of 160 prevalent HD patients from 10 centers across Spain were obtained from the Biobank of the Nephrology Renal Network (Madrid, Spain): 80 patients with DM and 80 patients without DM who were matched for clinical and demographic criteria. MVs from plasma and several monocyte subpopulations (CD142+/CD16+, CD14+/CD162+) were analyzed by flow cytometry, and the plasma concentrations of Ang1 and Ang2 were quantified by ELISA. Data on CVD were gathered over the 5.5 yr after these samples were obtained. MV level, monocyte subpopulations (CD14+/CD162+ and CD142+/CD16+), and Ang2-to-Ang1 ratios increased in HD patients with DM compared with non-DM patients. Moreover, MV level above the median (264 MVs/µl) was associated independently with greater mortality. MVs, monocyte subpopulations, and Ang2-to-Ang1 ratio can be used as predictors for CVD. In addition, MV level has a potential predictive value in the prevention of CVD in HD patients. These parameters undergo more extensive changes in patients with DM. Support for this work was provided by Plan Nacional de IDi Proyectos de Investigación en Salud of Instituto de Salud Carlos III (ISCIII)–Subdirección General de Evaluación, Fondos de desarrollo regional (FEDER; PI11/01536, PI12/01489, PI14/00806, PI15/01785); Junta de Andalucía grants (P010-CTS-6337, P11-CTS-7352); and Fundación Nefrológica. P. Buendía, A. Carmona, and C. Luna-Ruiz are fellows from Consejería de Innovacion, Ciencia y Empresa, Junta de Andalucía. American Physiological Society https://doi.org/10.1152/ajprenal.00013.2016 http://hdl.handle.net/10459.1/64518 |
| title |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis |
| spellingShingle |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis Carmona, Andrés Chronic kidney disease Cardiovascular disease Diabetes mellitus |
| title_short |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis |
| title_full |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis |
| title_fullStr |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis |
| title_full_unstemmed |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis |
| title_sort |
Markers of endothelial damage in patients with chronic kidney disease on hemodialysis |
| author |
Carmona, Andrés |
| author_facet |
Carmona, Andrés Agüera, Maria L. Luna-Ruiz, Carlos Buendía, Paula Calleros-Basilio, Laura García-Jerez, Andrea Rodríguez-Puyol, Manuel Arias, Manuel Arias-Guillen, Marta Arriba, Gabriel de Ballarín, José Bernis, Carmen Fernández i Giráldez, Elvira García-Rebollo, Sagrario Mancha, Javier Peso, Gloria del Pérez, Estefanía Poch, Esteban Portolés, José M. Rodríguez-Puyol, Diego Sánchez-Villanueva, Rafael Sarró, Felipe Torres, Armando Martín-Malo, Alejandro Aljama, Pedro Ramírez, Rafael Carracedo, Júlia |
| author_role |
author |
| author2 |
Agüera, Maria L. Luna-Ruiz, Carlos Buendía, Paula Calleros-Basilio, Laura García-Jerez, Andrea Rodríguez-Puyol, Manuel Arias, Manuel Arias-Guillen, Marta Arriba, Gabriel de Ballarín, José Bernis, Carmen Fernández i Giráldez, Elvira García-Rebollo, Sagrario Mancha, Javier Peso, Gloria del Pérez, Estefanía Poch, Esteban Portolés, José M. Rodríguez-Puyol, Diego Sánchez-Villanueva, Rafael Sarró, Felipe Torres, Armando Martín-Malo, Alejandro Aljama, Pedro Ramírez, Rafael Carracedo, Júlia |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author |
| topic |
Chronic kidney disease Cardiovascular disease Diabetes mellitus |
| topic_facet |
Chronic kidney disease Cardiovascular disease Diabetes mellitus |
| description |
Patients with Stage 5 chronic kidney disease who are on hemodialysis (HD) remain in a chronic inflammatory state, characterized by the accumulation of uremic toxins that induce endothelial damage and cardiovascular disease (CVD). Our aim was to examine microvesicles (MVs), monocyte subpopulations, and angiopoietins (Ang) to identify prognostic markers in HD patients with or without diabetes mellitus (DM). A total of 160 prevalent HD patients from 10 centers across Spain were obtained from the Biobank of the Nephrology Renal Network (Madrid, Spain): 80 patients with DM and 80 patients without DM who were matched for clinical and demographic criteria. MVs from plasma and several monocyte subpopulations (CD142+/CD16+, CD14+/CD162+) were analyzed by flow cytometry, and the plasma concentrations of Ang1 and Ang2 were quantified by ELISA. Data on CVD were gathered over the 5.5 yr after these samples were obtained. MV level, monocyte subpopulations (CD14+/CD162+ and CD142+/CD16+), and Ang2-to-Ang1 ratios increased in HD patients with DM compared with non-DM patients. Moreover, MV level above the median (264 MVs/µl) was associated independently with greater mortality. MVs, monocyte subpopulations, and Ang2-to-Ang1 ratio can be used as predictors for CVD. In addition, MV level has a potential predictive value in the prevention of CVD in HD patients. These parameters undergo more extensive changes in patients with DM. |
| publishDate |
2017 |
| format |
article |
| status_str |
acceptedVersion |
| url |
https://doi.org/10.1152/ajprenal.00013.2016 http://hdl.handle.net/10459.1/64518 |
| eu_rights_str_mv |
openAccess |
| publisher |
American Physiological Society |
| institution |
Universitat de Lleida (UdL) |
| collection |
Repositori Obert UdL |
| reponame_str |
Repositori Obert UdL |
| instname_str |
Universitat de Lleida (UdL) |
| _version_ |
1878443224876449792 |
| publishDateSort |
2017 |
| author_browse |
Agüera, Maria L. Aljama, Pedro Arias, Manuel Arias-Guillen, Marta Arriba, Gabriel de Ballarín, José Bernis, Carmen Buendía, Paula Calleros-Basilio, Laura Carmona, Andrés Carracedo, Júlia Fernández i Giráldez, Elvira García-Jerez, Andrea García-Rebollo, Sagrario Luna-Ruiz, Carlos Mancha, Javier Martín-Malo, Alejandro Peso, Gloria del Poch, Esteban Portolés, José M. Pérez, Estefanía Ramírez, Rafael Rodríguez-Puyol, Diego Rodríguez-Puyol, Manuel Sarró, Felipe Sánchez-Villanueva, Rafael Torres, Armando |
| publisherStr |
American Physiological Society |
| score |
6.924472 |