Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids

Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular...

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Autores: Francisco Guillén-Chable, ULISES RODRIGUEZ CORONA, Alejandro Pereira-Santana, ANDREA BAYONA HERNANDEZ, Luis Carlos Rodríguez Zapata, Cecilia Aquino Perez, Lenka Šebestová, Nicolas Vitale, Pavel Hozak, Enrique Castaño de la Serna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:México
Institución:Centro de Investigación Científica de Yucatán
Repositorio:Repositorio Institucional CICY
Idioma:inglés
OAI Identifier:oai:cicy.repositorioinstitucional.mx:1003/1822
Acceso en línea:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
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oai_identifier_str oai:cicy.repositorioinstitucional.mx:1003/1822
network_acronym_str MX
network_name_str México
spelling Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids Francisco Guillén-Chable ULISES RODRIGUEZ CORONA Alejandro Pereira-Santana ANDREA BAYONA HERNANDEZ Luis Carlos Rodríguez Zapata Cecilia Aquino Perez Lenka Šebestová Nicolas Vitale Pavel Hozak Enrique Castaño de la Serna info:eu-repo/classification/Autores/NUCLEOLUS info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE info:eu-repo/classification/Autores/RRNA info:eu-repo/classification/Autores/FIBRILLARIN info:eu-repo/classification/Autores/PHOSPHOINOSITIDES info:eu-repo/classification/Autores/VIRAL PROGRESSION info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
title Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
spellingShingle Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
Francisco Guillén-Chable
info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
title_short Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_full Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_fullStr Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_full_unstemmed Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
title_sort Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids
author Francisco Guillén-Chable
author_facet Francisco Guillén-Chable
ULISES RODRIGUEZ CORONA
Alejandro Pereira-Santana
ANDREA BAYONA HERNANDEZ
Luis Carlos Rodríguez Zapata
Cecilia Aquino Perez
Lenka Šebestová
Nicolas Vitale
Pavel Hozak
Enrique Castaño de la Serna
author_role author
author2 ULISES RODRIGUEZ CORONA
Alejandro Pereira-Santana
ANDREA BAYONA HERNANDEZ
Luis Carlos Rodríguez Zapata
Cecilia Aquino Perez
Lenka Šebestová
Nicolas Vitale
Pavel Hozak
Enrique Castaño de la Serna
author2_role author
author
author
author
author
author
author
author
author
topic info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
topic_facet info:eu-repo/classification/Autores/NUCLEOLUS
info:eu-repo/classification/Autores/RIBONUCLEOLAR PARTICLE
info:eu-repo/classification/Autores/RRNA
info:eu-repo/classification/Autores/FIBRILLARIN
info:eu-repo/classification/Autores/PHOSPHOINOSITIDES
info:eu-repo/classification/Autores/VIRAL PROGRESSION
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2415
info:eu-repo/classification/cti/241502
description Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli.
publishDate 2020
format article
status_str publishedVersion
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822
language eng
eu_rights_str_mv openAccess
institution CICY
collection Repositorio Institucional CICY
reponame_str Repositorio Institucional CICY
instname_str Centro de Investigación Científica de Yucatán
_version_ 1878409813088534528
publishDateSort 2020
author_browse ANDREA BAYONA HERNANDEZ
Alejandro Pereira-Santana
Cecilia Aquino Perez
Enrique Castaño de la Serna
Francisco Guillén-Chable
Lenka Šebestová
Luis Carlos Rodríguez Zapata
Nicolas Vitale
Pavel Hozak
ULISES RODRIGUEZ CORONA
score 6,9303427

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