Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don
Brunfelsia grandiflora D. Don “chiric sanango” is a plant native to the Peruvian Amazon, belonging to the Solanaceae and is used in traditional medicine by the jungle population. The objective of the research was to determine the content of total polyphenols and the in vitro antioxidant activity of...
| Autores: | , , |
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| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2022 |
| País: | Perú |
| Recursos: | Universidad Nacional Mayor de San Marcos |
| Repositório: | Revistas - Universidad Nacional Mayor de San Marcos |
| Idioma: | espanhol |
| OAI Identifier: | oai:revistasinvestigacion.unmsm.edu.pe:article/23476 |
| Acesso em linha: | https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/23476 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Antioxidant Brunfelsia grandiflora D. Don total polyphenols Antioxidante polifenoles totales |
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oai:revistasinvestigacion.unmsm.edu.pe:article/23476 |
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Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don Polifenoles totales y actividad antioxidante in vitro del extracto acuoso de la corteza de Brunfelsia grandiflora D. Don Giraldo, Leonardo Ramos, Norma Castro, Américo Antioxidant Brunfelsia grandiflora D. Don total polyphenols Antioxidante polifenoles totales Brunfelsia grandiflora D. Don “chiric sanango” is a plant native to the Peruvian Amazon, belonging to the Solanaceae and is used in traditional medicine by the jungle population. The objective of the research was to determine the content of total polyphenols and the in vitro antioxidant activity of the aqueous extract of its bark. The quantification of total polyphenols was carried out by applying the Folin-Ciocalteu method and the antioxidant activity by the method of elimination of free radical by electron transfer using 2,2-diphenyl-1-picrylhydrazyl (DPPH) the capacity method ferric reducing antioxidant (FRAP) and the hydrogen transfer method using 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS*†). The content of total polyphenols was 30,17 mg of gallic acid/100g of the extract and the antioxidant activity by the DPPH method was 11,86 mg Trolox/g sample; by the FRAP method it was 113,9 µmol of ascorbic acid equivalents/g sample and by the ABTS*† method it was 80,38 mg Trolox/g sample. Therefore, it can be concluded that the aqueous extract is an accessible source of antioxidants. Brunfelsia grandiflora D. Don “chiric sanango” es una planta oriunda de la amazonia peruana, perteneciente a las solanáceas y es utilizada en la medicina tradicional por la población selvática. La investigación tuvo como objetivo determinar el contenido de polifenoles totales y la actividad antioxidante in vitro del extracto acuoso de su corteza. La cuantificación de los polifenoles totales se realizó aplicando el método de Folin-Ciocalteu y la actividad antioxidante por el método de eliminación de radicales libres por transferencia de electrones empleando el 2,2-difenil-1-picrilhidrazil (DPPH), el método de capacidad antioxidante reductor férrico (FRAP) y el método por transferencia de hidrógeno empleando el ácido 2,2-azino-bis 3-etilbenzotiazolina-6-sulfónico (ABTS*†). El contenido de polifenoles totales fue de 30,17 mg de ácido gálico/100g del extracto y la actividad antioxidante por el método DPPH fue de 11,86 mg Trolox/g muestra; por el método FRAP fue de 113,9 µmol de equivalentes de ácido ascórbico/g de muestra y por el método ABTS*† de 80,38 mg Trolox/g muestra. Por lo que se puede concluir que el extracto acuoso es una fuente accesible de antioxidantes. Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/23476 10.15381/ci.v25i1.23476 |
| title |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don |
| spellingShingle |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don Giraldo, Leonardo Antioxidant Brunfelsia grandiflora D. Don total polyphenols Antioxidante polifenoles totales |
| title_short |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don |
| title_full |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don |
| title_fullStr |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don |
| title_full_unstemmed |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don |
| title_sort |
Total polyphenols and in vitro antioxidant activity of the aqueous extract of the bark of Brunfelsia grandiflora D. Don |
| author |
Giraldo, Leonardo |
| author_facet |
Giraldo, Leonardo Ramos, Norma Castro, Américo |
| author_role |
author |
| author2 |
Ramos, Norma Castro, Américo |
| author2_role |
author author |
| topic |
Antioxidant Brunfelsia grandiflora D. Don total polyphenols Antioxidante polifenoles totales |
| topic_facet |
Antioxidant Brunfelsia grandiflora D. Don total polyphenols Antioxidante polifenoles totales |
| description |
Brunfelsia grandiflora D. Don “chiric sanango” is a plant native to the Peruvian Amazon, belonging to the Solanaceae and is used in traditional medicine by the jungle population. The objective of the research was to determine the content of total polyphenols and the in vitro antioxidant activity of the aqueous extract of its bark. The quantification of total polyphenols was carried out by applying the Folin-Ciocalteu method and the antioxidant activity by the method of elimination of free radical by electron transfer using 2,2-diphenyl-1-picrylhydrazyl (DPPH) the capacity method ferric reducing antioxidant (FRAP) and the hydrogen transfer method using 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS*†). The content of total polyphenols was 30,17 mg of gallic acid/100g of the extract and the antioxidant activity by the DPPH method was 11,86 mg Trolox/g sample; by the FRAP method it was 113,9 µmol of ascorbic acid equivalents/g sample and by the ABTS*† method it was 80,38 mg Trolox/g sample. Therefore, it can be concluded that the aqueous extract is an accessible source of antioxidants. |
| publishDate |
2022 |
| format |
article |
| status_str |
publishedVersion |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/23476 |
| identifier_str_mv |
10.15381/ci.v25i1.23476 |
| language |
spa |
| eu_rights_str_mv |
openAccess |
| publisher |
Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica |
| institution |
UNMSM |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| _version_ |
1878413192294563841 |
| publishDateSort |
2022 |
| author_browse |
Castro, Américo Giraldo, Leonardo Ramos, Norma |
| publisherStr |
Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica |
| score |
6.924472 |